Preparation of an amino acid-based polymer monolith for trimodal liquid chromatography

被引：8

作者：

Li, Nan ^{[1
,2
]}

Shen, Yong ^{[1
,2
]}

Qi, Li ^{[1
]}

Li, Zhibo ^{[1
]}

Qiao, Juan ^{[1
]}

Chen, Yi ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[2] Shandong Agr Univ, Grad Sch, Shandong 271018, Peoples R China

来源：

RSC ADVANCES | 2015年 / 5卷 / 75期

基金：

中国国家自然科学基金;

关键词：

RADICAL POLYMERIZATION; SMALL MOLECULES; SEPARATION; COLUMN;

D O I：

10.1039/c5ra12203a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A versatile method for preparing a reverse phase/weak anion exchange/hydrophilic interaction trimodal polymer monolith was proposed by the one-step copolymerization of amino acid-based monomer and N,N'-methylenebisacrylamide. The retention mechanisms of the resultant monolith were evaluated using different series of small molecules. Furthermore, the separation of protein mixtures could be easily achieved under isocratic elution. The proposed polymer monolith, which has the merits of easy preparation, low mass transfer resistance and good biocompatibility, is a promising separation medium for small and large molecules.

引用

页码：61436 / 61439

页数：4

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