Role of calcium ions and temperature on dextransucrase production

被引：0

作者：

Qader, Shah Ali Ul ^{[1
]}

Aman, Afsheen ^{[1
]}

Bano, Saeeda ^{[1
]}

Syed, Noman ^{[1
]}

Azhar, Abid ^{[2
]}

机构：

[1] PCSIR Labs Complex, Pharmaceut Res Ctr, Karachi, Pakistan

[2] Univ Karachi, Inst Biotechnol & Genet Engn, Karachi, Pakistan

来源：

INDIAN JOURNAL OF BIOTECHNOLOGY | 2008年 / 7卷 / 03期

关键词：

dextran; CaCl2; stability; dextransucrase; glucosyltransferase; Leuconostoc mesenteroides;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dextransucrase [E.C. 2.4.1.5] enzyme from a newly isolated strain of Leuconostoc mesenteroides PCSIR-4 showed maximum production in a fermentation medium containing 0.005% CaCl2. The production of dextransucrase was 2.5 times higher in the medium containing calcium ions as compared to the medium without calcium ions. Also, the stability of the enzyme increased up to 120 d. Maximum dextransucrase production in fermentation medium was obtained when culture was incubated at 25 degrees C, while the maximum extracellular enzyme activity was observed at 35 degrees C. Thermal stability of extracellular enzyme was maximum at 30 degrees C, while at 50 degrees C the total enzyme loss was observed in 240 min.

引用

页码：404 / 406

页数：3

共 24 条

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