Identification of type II toxin-antitoxin modules in Burkholderia pseudomallei

被引：20

作者：

Butt, Aaron ^{[1
]}

Mueller, Claudia ^{[1
]}

Harmer, Nicholas ^{[1
]}

Titball, Richard W. ^{[1
]}

机构：

[1] Univ Exeter, Coll Life & Environm Sci, Dept Biosci, Exeter EX4 4QD, Devon, England

来源：

FEMS MICROBIOLOGY LETTERS | 2013年 / 338卷 / 01期

基金：

英国惠康基金;

关键词：

toxin-antitoxin; Burkholderia pseudomallei; melioidosis; persister cell; antibiotic resistance; expression and screening system; MEDIATED CELL-DEATH; ESCHERICHIA-COLI; PERSISTER CELLS; BACTERIA; SYSTEMS; TOLERANCE; GENES; LOCI;

D O I：

10.1111/1574-6968.12032

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Type II toxinantitoxin (TA) systems are believed to be widely distributed amongst bacteria although their biological functions are not clear. We have identified eight candidate TA systems in the genome of the human pathogen Burkholderia pseudomallei. Five of these were located in genome islands. Of the candidate toxins, BPSL0175 (RelE1) or BPSS1060 (RelE2) caused growth to cease when expressed in Escherichia coli, whereas expression of BPSS0390 (HicA) or BPSS1584 (HipA) (in an E. coli Delta hipBA background) caused a reduction in the number of culturable bacteria. The cognate antitoxins could restore growth and culturability of cells.

引用

页码：86 / 94

页数：9

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