Expression pattern and localization of alpha-synuclein in the human enteric nervous system

被引：108

作者：

Boettner, Martina ^{[1
]}

Zorenkov, Dimitri ^{[2
]}

Hellwig, Ines ^{[1
]}

Barrenschee, Martina ^{[1
]}

Harde, Jonas ^{[1
]}

Fricke, Tobias ^{[1
]}

Deuschl, Guenther ^{[2
]}

Egberts, Jan-Hendrik ^{[3
]}

Becker, Thomas ^{[3
]}

Fritscher-Ravens, Annette ^{[4
]}

Arlt, Alexander ^{[4
]}

Wedel, Thilo ^{[1
]}

机构：

[1] Univ Kiel, Dept Anat, D-24118 Kiel, Germany

[2] Univ Hosp Schleswig Holstein, Dept Neurol, D-24105 Kiel, Germany

[3] Univ Hosp Schleswig Holstein, Dept Gen & Thorac Surg, D-24105 Kiel, Germany

[4] Univ Hosp Schleswig Holstein, Dept Internal Med 1, D-24105 Kiel, Germany

来源：

NEUROBIOLOGY OF DISEASE | 2012年 / 48卷 / 03期

关键词：

alpha-synuclein; Enteric nervous system; Neuropathies; Human colon; PARKINSONS-DISEASE; LEWY BODIES; MONOCLONAL-ANTIBODY; COLONIC BIOPSIES; HUMAN BRAIN; PATHOLOGY; DEMENTIA; NEURONS; AGGREGATION; INCLUSIONS;

D O I：

10.1016/j.nbd.2012.07.018

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Background: Alpha-synuclein (alpha-syn) is abundantly expressed in the central nervous system and involved in the regulation of neurotransmission. Insoluble fibrils of phosphorylated a-synuclein (p-alpha-syn) have been implicated in several neurodegenerative diseases (e.g. Parkinson's disease, Alzheimer's disease). The aim of the study was to determine the gene expression pattern and localization of alpha-syn/p-alpha-syn in the human enteric nervous system (ENS). Methods: Human colonic specimens (n = 13, 15-83 years) were processed for alpha-syn and p-alpha-syn immunohistochemistry. Colocalization of alpha-syn was assessed by dual-labeling with pan-neuronal markers (PGP 9.5, HuC/D). For qPCR studies, tissue was obtained from full-thickness sections, tunica muscularis, submucosa, mucosa, and laser-microdissected (LMD) enteric ganglia. Results: Highest alpha-syn levels were detectable within the tunica muscularis and submucosa. Ganglia isolated by LMD showed high expression of alpha-syn mRNA. All myenteric and submucosal ganglia and nerve fibers were immunoreactive for alpha-syn. Dual-labeling revealed colocalization of alpha-syn with both pan-neuronal markers. p-alpha-syn immunoreactivity was consistently observed in specimens from adults with increasing age. Conclusions: alpha-syn is abundantly expressed in all nerve plexus of the human ENS including both neuronal somata and processes. The presence of p-alpha-syn within the ENS is a regular finding in adults with increasing age and may not be regarded as pathological correlate. The data provide a basis to unravel the functions of alpha-syn and to evaluate altered alpha-syn in enteric neuropathies and alpha-synucleinopathies of the CNS with gastrointestinal manifestations. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：474 / 480

页数：7

共 28 条

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