Structure and anticancer activity of sulfated O-polysaccharide from marine bacterium Cobetia litoralis KMM 3880T

被引：27

作者：

Kokoulin, Maxim S. ^{[1
]}

Kuzmich, Alexandra S. ^{[1
]}

Kalinovsky, Anatoly I. ^{[1
]}

Tomshich, Svetlana V. ^{[1
]}

Romanenko, Lyudmila A. ^{[1
]}

Mikhailov, Valery V. ^{[1
,2
]}

Komandrova, Nadezhda A. ^{[1
]}

机构：

[1] Russian Acad Sci, GB Elyakov Pacific Inst Bioorgan Chem, Far Eastern Branch, 100 Let Vladivostoku Prosp,159, Vladivostok 690022, Russia

[2] Far Eastern Fed Univ, Suhanova St 8, Vladivostok 690950, Russia

来源：

CARBOHYDRATE POLYMERS | 2016年 / 154卷

关键词：

Marine bacterium; Cobetia litoralis; Lipopolysaccharide; O-polysaccharide; Sulfated 3-deoxy-D-manno-oct-2-ulosonic acid; Anticancer activity; H-1-NMR; SPECTRA; ANTIGEN; ESTERS;

D O I：

10.1016/j.carbpol.2016.08.036

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

We presented the structure of the polysaccharide moiety and anticancer activity in vitro of the sulfated lipopolysaccharide isolated from the marine bacterium Cobetia litoralis KMM 3880(T). The structure of O-polysaccharide was investigated by chemical methods along with H-1 and C-13 NMR spectroscopy. The O-polysaccharide was built up of branched trisaccharide repeating units consist of D-glucose (D-Glcp), D-mannose (D-Manp) and sulfated 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo5S): >7-beta-Kdop4Ac5S-(2>4)-[beta-D-Glcp-(1>2)-]-beta-D-Manp6Ac-1>. We demonstrated that the lipopolysaccharide and 0-deacetylated O-polysaccharide from Cobetia litoralis KMM 3880(T) inhibited a colony formation of human melanoma SK-MEL-28 and colorectal carcinoma HTC-116 cells. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：55 / 61

页数：7

共 16 条

[1] 5-(3-CARBOXYMETHOXYPHENYL)-2-(4,5-DIMETHYLTHIAZOLYL)-3-(4-SULFOPHENYL)TETRAZOLIUM, INNER SALT (MTS) AND RELATED ANALOGS OF 3-(4,5-DIMETHYLTHIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) REDUCING TO PURPLE WATER-SOLUBLE FORMAZANS AS CELL-VIABILITY INDICATORS [J].

BARLTROP, JA ;

OWEN, TC ;

CORY, AH ;

CORY, JG .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1991, 1 (11) :611-&

[2] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES [J].

BOCK, K ;

PEDERSEN, C .

ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1983, 41 :27-66

[3] DISSOCIATION OF MITOGENESIS AND LATE-STAGE PROMOTION OF TUMOR-CELL PHENOTYPE BY PHORBOL ESTERS - MITOGEN-RESISTANT VARIANTS ARE SENSITIVE TO PROMOTION [J].

COLBURN, NH ;

WENDEL, EJ ;

ABRUZZO, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11) :6912-6916

[4] DETERMINATION OF INORGANIC SULPHATE IN STUDIES ON ENZYMIC AND NON-ENZYMIC HYDROLYSIS OF CARBOHYDRATE AND OTHER SULPHATE ESTERS [J].

DODGSON, KS .

BIOCHEMICAL JOURNAL, 1961, 78 (02) :312-&

[5] Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects [J].

Fedorov, Sergey N. ;

Ermakova, Svetlana P. ;

Zvyagintseva, Tatyana N. ;

Stonik, Valentin A. .

MARINE DRUGS, 2013, 11 (12) :4876-4901

[6] COMPUTER-ASSISTED STRUCTURAL-ANALYSIS OF POLYSACCHARIDES WITH AN EXTENDED VERSION OF CASPER USING H-1-NMR AND C-13-NMR DATA [J].

JANSSON, PE ;

KENNE, L ;

WIDMALM, G .

CARBOHYDRATE RESEARCH, 1989, 188 :169-191

[7] The new sulfated O-specific polysaccharide from marine bacterium Cobetia pacifica KMM 3878, containing 3,4-O-[(S)-1-carboxyethylidene]-D-galactose and 2,3-O-disulfate-D-galactose [J].

Kokoulin, Maxim S. ;

Kalinovsky, Anatoliy I. ;

Komandrova, Nadezhda A. ;

Tomshich, Svetlana V. ;

Romanenko, Lyudmila A. ;

Vaskovsky, Victor E. .

CARBOHYDRATE RESEARCH, 2014, 397 :46-51

[8] The sulfated O-specific polysaccharide from the marine bacterium Cobetia pacifica KMM 3879T [J].

Kokoulin, Maxim S. ;

Kalinovsky, Anatoliy I. ;

Komandrova, Nadezhda A. ;

Tomshich, Svetlana V. ;

Romanenko, Lyudmila A. ;

Vaskovsky, Victor E. .

CARBOHYDRATE RESEARCH, 2014, 387 :4-9

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] The marine bacteria Cobetia marina DSMZ 4741 synthesizes an unexpected K-antigen-like exopolysaccharide [J].

Lelchat, Florian ;

Cerantola, Stephane ;

Brandily, Christophe ;

Colliec-Jouault, Sylvia ;

Baudoux, Anne-Claire ;

Ojima, Takao ;

Boisset, Claire .

CARBOHYDRATE POLYMERS, 2015, 124 :347-356

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