Preparation and drug release behavior of amphiphilic polyamino acids nanomicelles

To construct pH sensitive nanomicelles as novel drug delivery carriers, the synthesis of four triblock copolymer mPEG113-Lysinen-Leucine10 based on methoxypolyethylene glycol, lysine and leucine is described, and the structure of copolymer were characterized by FT-IR and 1H NMR. The polymer can be self-assembled into spherical or cubic shape in aqueous solution, and TEM revealed that the drug-loaded nanomicelles displayed uniformly dispersed spherical shape. The average particle size of nanomicelles is about 200 nm, which meets the requirements of material size in the field of sustained release. CCK-8 experiment proved that the synthesized material had good cytocompatibility, and the drug-loaded nanomicelles could be successfully uptaken by Hela cells, and model drug could be successfully released in cytoplasm and nucleus. Uv-vis results showed that the in vitro drug release of the nanomicelles was pH responsive and showed good dimensional stability at different temperatures.