Terrimonas pekingensis sp nov., isolated from bulking sludge, and emended descriptions of the genus Terrimonas, Terrimonas ferruginea, Terrimonas lutea and Terrimonas aquatica

A Gram-negative, strictly aerobic, non-motile and non-spore-forming rod that produced white, viscous colonies, designated QH(T), was isolated from bulking sludge collected from a municipal wastewater treatment plant in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QH(T) belonged to the genus Terrimonas and shared 96.3% sequence similarity with Terrimonas lutea DYT, 94.1% with Terrimonas ferruginea ATCC 13524(T) and 93.8 % with Terrimonas aquatica RIB1-6(T). Strain QH(T) contained iso-C-15:0, summed feature 3 (comprising one or more of C-16:1 omega 7c, C-16:1 omega 6c and iso-C-15:0 2-OH) and iso-C-15:1 G as the predominant fatty acids. The predominant polar lipid of strain QH(T) and members of the genus Terrimonas was phosphatidylethanolamine. The major isoprenoid quinone of strain QH(T) was MK-7 and the DNA G+C content was 41.0 mol%. DNA-DNA relatedness between strain QH(T) and T. lutea BCRC 17944(T), T. ferruginea BCRC 17943(T) and T. aquatica BCRC 17941(T) was 32, 23 and 22%, respectively. On the basis of phylogenetic inference, differential phenotypic data and low DNA-DNA relatedness with members of the genus Terrimonas, strain QH(T) represents a novel species, for which the name Terrimonas pekingensis sp. nov. is proposed. The type strain is QH(T) (=CICC 10452(T) =NCCB 100397(T)). The descriptions of the genus Terrimonas and T. ferruginea, T. lutea and T. aquatica are also emended.