Size-exclusion chromatography - a review of calibration methodologies

被引：130
|
作者：
Kostanski, LK [1 ]
Keller, DM [1 ]
Hamielec, AE [1 ]
机构：
[1] McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L7, Canada
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 58卷 / 02期
关键词：
size exclusion chromatography; gel permeation chromatography; gel filtration; calibration; methodologies; direct calibration; universal calibration;
D O I：
10.1016/j.jbbm.2003.10.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent developments are reviewed in size exclusion chromatographic calibration methodologies, including direct calibration by using narrow and broad polymer standards and various instrumental methods (nuclear magnetic resonance, mass spectrometry, light scattering) as well as universal calibration with and without viscometry detectors, for simple and complex polymers. (C) 2003 Elsevier B.V. All rights reserved.
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页码：159 / 186
页数：28
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