Characterization of Escherichia coli O78 from an outbreak of septicemia in lambs in Norway

被引：12

作者：

Kjelstrup, Cecilie K. ^{[1
]}

Arnesen, Lotte P. Stenfors ^{[1
]}

Granquist, Erik G. ^{[2
]}

L'Abee-Lund, Trine M. ^{[1
]}

机构：

[1] Norwegian Sch Vet Sci, Dept Food Safety & Infect Biol, N-0033 Oslo, Norway

[2] Norwegian Sch Vet Sci, Dept Prod Anim Clin Sci, N-0033 Oslo, Norway

来源：

VETERINARY MICROBIOLOGY | 2013年 / 166卷 / 1-2期

关键词：

Escherichia coli; Septicemia; Sheep; Neonatal; Virulence; EXTRAINTESTINAL PATHOGENIC ISOLATE; VIRULENCE GENE; STRAINS;

D O I：

10.1016/j.vetmic.2013.05.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of the study was to characterize isolates of Escherichia coli from an outbreak of septicemia in a Norwegian sheep flock in 2008 with emphasis on virulence, serological grouping, phylogenicity and homology. Six E. coli isolates from succumbed neonatal lambs and four E. coli isolates collected from healthy individuals were analyzed by Pulsed-Field Gel Electrophoresis (PFGE), miniaturized microarray, and polymerase chain reaction (PCR). The septicemic E. coli isolates showed identical pulsotypes (PTs), and belonged to serogroup O78, phylogenetic group A, and MLST ST 369. The virulence genes f17G, bmaE, afaE-VIII, ireA, iroN and iss were detected in the septicemic isolates. The results showed that the E. coli isolates from the septicemic outbreak had a clonal appearance, thus likely originating from a common source. The clone carried genes important for virulence, however, a significant explanation for the high pathogenicity was not revealed. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：276 / 280

页数：5

共 20 条

[1] Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine [J].

Chapman, Toni A. ;

Wu, Xi-Yang ;

Barchia, Idris ;

Bettelheim, Karl A. ;

Driesen, Steven ;

Trott, Darren ;

Wilson, Mark ;

Chin, James J. -C. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (07) :4782-4795

[2] Rapid and simple determination of the Escherichia coli phylogenetic group [J].

Clermont, O ;

Bonacorsi, S ;

Bingen, E .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4555-4558

[3] CHARACTERS OF ESCHERICHIA-COLI 078 ISOLATED FROM SEPTICEMIC ANIMALS [J].

DASSOULIMRANIBELKEBIR, A ;

CONTREPOIS, M ;

GIRARDEAU, JP ;

DERVARTANIAN, M .

VETERINARY MICROBIOLOGY, 1988, 17 (04) :345-356

[4] Colibacillosis in poultry:: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts [J].

Dziva, Francis ;

Stevens, Mark P. .

AVIAN PATHOLOGY, 2008, 37 (04) :355-366

[5] Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day [J].

Gautom, RK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (11) :2977-2980

[6] The afa-related gene cluster in necrotoxigenic and other Escherichia coli from animals belongs to the afa-8 variant [J].

Gérardin, J ;

Lalioui, L ;

Jacquemin, E ;

Le Bouguénec, C ;

Mainil, JG .

VETERINARY MICROBIOLOGY, 2000, 76 (02) :175-184

[7] Characteristics and virulence genes of Escherichia coli isolated from septicemic calves in southeast of Iran [J].

Ghanbarpour, Reza ;

Oswald, Eric .

TROPICAL ANIMAL HEALTH AND PRODUCTION, 2009, 41 (07) :1091-1099

[8] Assigning Escherichia coli strains to phylogenetic groups:: multi-locus sequence typing versus the PCR triplex method [J].

Gordon, David M. ;

Clermont, Olivier ;

Tolley, Heather ;

Denamur, Erick .

ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (10) :2484-2496

[9]

Hodgson J. C., 1994, P135

[10] The lpf operon of invasive Escherichia coli [J].

Ideses, D ;

Biran, D ;

Gophna, U ;

Levy-Nissenbaum, O ;

Ron, EZ .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 295 (04) :227-236

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