Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

被引：67

作者：

de la Cruz-Hernandez, Sergio I. ^{[1
]}

Flores-Aguilar, Hilario ^{[1
]}

Gonzalez-Mateos, Silvia ^{[1
]}

Lopez-Martinez, Irma ^{[1
]}

Alpuche-Aranda, Celia ^{[1
]}

Ludert, Juan E. ^{[2
]}

del Angel, Rosa M. ^{[2
]}

机构：

[1] Inst Diagnost & Referencia Epidemiol, Mexico City 11340, DF, Mexico

[2] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Infect & Patogenesis Mol, Mexico City 07360, DF, Mexico

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2013年 / 88卷 / 03期

关键词：

NS1; SEROTYPE; DIAGNOSIS; CORRELATE; DISEASE; ANTIGEN; MARKERS; GLYCOPROTEIN; PATHOGENESIS; KINETICS;

D O I：

10.4269/ajtmh.12-0023

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Higher levels of viremia and circulating nonstructural protein 1 (NS1) have been associated with dengue disease severity. In this study, viremia and circulating NS1 levels were determined in 225 serum samples collected from patients in Mexico infected with dengue virus serotypes 1 and 2 (DENV-1 and DENV-2). Patients with dengue hemorrhagic fever (DHF) who were infected with DENV-1 showed higher levels of circulating NS1 than patients with dengue fever (DF) (P = 0.0175). Moreover, NS1 levels were higher in patients with primary infections with DENV-1 than in patient infected with DENV-2 (P < 0.0001) and in patients with primary infections with DENV-2 than in patients with secondary infections with DENV-2 (P = 0.0051). Unexpectedly, viremia levels were higher in patients with DF than in those with DHF infected with either DENV-1 or DENV-2 (P = 0.0019 and P = 0.001, respectively) and in patients with primary infections than those with secondary DENV-2 infections (P < 0.0001). Results indicate that levels of circulating NS1 vary according to the infecting serotype, immunologic status (primary or secondary infection), and dengue disease severity.

引用

页码：446 / 454

页数：9

共 38 条

[1] Evaluation of ASSURE® Dengue IgA Rapid Test using dengue-positive and dengue-negative samples [J].

Ahmed, Firoz ;

Mursalin, Huzzatul ;

Alam, Meer Taifur ;

Amin, Ruhul ;

Sekaran, Shamala Devi ;

Wang, Seok Mui ;

Tan, Yun Ying ;

Sil, Bijon Kumar ;

Hossain, M. Anowar .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2010, 68 (04) :339-344

[2] Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the experiencing primary acute phase of disease in patients or secondary infections [J].

Alcon, S ;

Talarmin, A ;

Debruyne, M ;

Falconar, A ;

Deubel, V ;

Flamand, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (02) :376-381

[3] Polyclonal antibodies against properly folded Dengue virus NS1 protein expressed in E-coli enable sensitive and early dengue diagnosis [J].

Allonso, Diego ;

Rosa, Marcela da Silva ;

Coelho, Diego Rodrigues ;

da Costa, Simone Morais ;

Ribeiro Nogueira, Rita Maria ;

Bozza, Fernando Augusto ;

Dos Santos, Flavia Barreto ;

de Barcelos Alves, Ada Maria ;

Mohana-Borges, Ronaldo .

JOURNAL OF VIROLOGICAL METHODS, 2011, 175 (01) :109-116

[4] Vascular leakage in severe dengue virus infections: A potential role for the nonstructural viral protein NS1 and complement [J].

Avirutnan, P ;

Punyadee, N ;

Noisakran, S ;

Komoltri, C ;

Thiemmeca, S ;

Auethavornanan, K ;

Jairungsri, A ;

Kanlaya, R ;

Tangthawornchaikul, N ;

Puttikhunt, C ;

Pattanakitsakul, SN ;

Yenchitsomanus, PT ;

Mongkolsapaya, J ;

Kasinrerk, W ;

Sittisombut, N ;

Husmann, M ;

Blettner, M ;

Vasanawathana, S ;

Bhakdi, S ;

Malasit, P .

JOURNAL OF INFECTIOUS DISEASES, 2006, 193 (08) :1078-1088

[5] Evaluation of immunological markers in serum, filter-paper blood spots, and saliva for dengue diagnosis and epidemiological studies [J].

Balmaseda, Angel ;

Saborio, Saira ;

Tellez, Yolanda ;

Mercado, Juan Carlos ;

Perez, Leone ;

Hammond, Samantha N. ;

Rocha, Crisanta ;

Kuan, Guillermina ;

Harris, Eva .

JOURNAL OF CLINICAL VIROLOGY, 2008, 43 (03) :287-291

[6] Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection [J].

Bessoff, Kovi ;

Delorey, Mark ;

Sun, Wellington ;

Hunsperger, Elizabeth .

CLINICAL AND VACCINE IMMUNOLOGY, 2008, 15 (10) :1513-1518

[7] Recent advances in deciphering viral and host determinants of dengue virus replication and pathogenesis [J].

Clyde, Karen ;

Kyle, Jennifer L. ;

Harris, Eva .

JOURNAL OF VIROLOGY, 2006, 80 (23) :11418-11431

[8] Dengue vaccines: progress and challenges [J].

Coller, Beth-Ann G. ;

Clements, David E. .

CURRENT OPINION IN IMMUNOLOGY, 2011, 23 (03) :391-398

[9] Humoral immune response of dengue hemorrhagic fever cases in children from Tegucigalpa, Honduras [J].

De Rivera, Ivette Lorenzana ;

Parham, Leda ;

Murillo, Wendy ;

Moncada, Walter ;

Vazquez, Susana .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2008, 79 (02) :262-266

[10] Clinical and Virological Factors Influencing the Performance of a NS1 Antigen-Capture Assay and Potential Use as a Marker of Dengue Disease Severity [J].

Duong, Veasna ;

Ly, Sowath ;

Try, Patrich Lorn ;

Tuiskunen, Anne ;

Ong, Sivuth ;

Chroeung, Norith ;

Lundkvist, Ake ;

Leparc-Goffart, Isabelle ;

Deubel, Vincent ;

Vong, Sirenda ;

Buchy, Philippe .

PLOS NEGLECTED TROPICAL DISEASES, 2011, 5 (07)

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