Leaf chlorophyll concentration relates to transpiration efficiency in peanut

Two pot experiments were conducted ill two different seasons at the University of Agricultural Science, Bangalore, India, 10 study (a) the relationship between chlorophyll concentration (by measuring the leaf light-transmittance characteristics using a SPAD metre) and transpiration efficiency (TE) and (b) the effect of leaf N on chlorophyll and TE relationship in peanut. In Experiment (Expt) 1, six peanut genotypes with wide genetic variation for the specific leaf area (SLA) were Used. In Expt 11, three non-nodulating isogenic lines were used to study the effect of N levels oil leaf chlorophyll concentration-TE relationship without potential confounding effects in biological nitrogen fixation. Leaf N was manipulated by applying N fertiliser in Expt II. Chlorophyll concentration, TE (g dry matter kg(-1) of H2O transpired, measured using gravimetric method), specific leaf nitrogen (g N m(-2), SLN), SLA (cm(2) g(-1)), carbon isotope composition (Delta C-13) were determined in the leaves sampled during the treatment period (35-55 days after sowing) in the two experiments. Results showed that the leaf chlorophyll concentration expressed as soil plant analytical development (SPAD) chlorophyll metre reading (SCMR) varied significantly among genotypes in Expt I and as a result of N application in Expt II. Changes ill leaf N levels were strongly associated with changes in SCMR, TE and Delta C-13. In both the experiments, a significant positive relationship between SCMR and TE with similar slopes but differing intercepts was noticed. However, correction of TE for seasonal differences in vapour pressure deficit (VPD) between the two experiments resulted in a single and stronger relationship between SCMR and TE. There was a significant inverse relationship between SCMR and Delta C-13, suggesting a close linkage between chlorophyll concentration and Delta C-13 ill peanut. This study provides the First evidence for a significant positive relationship between TE and leaf chlorophyll concentration in peanut. The study also describes the effect of growing environment oil the relationships among SLA, SLN and SCMR.