A novel bi-functional DNA vaccine expressing VP1 protein and producing antisense RNA targeted to 5′UTR of foot-and-mouth disease virus can induce both rapid inhibitory effect and specific immune response in mice

被引：18

作者：

Yang, Bin ^{[1
,2
]}

Lan, Xi ^{[2
]}

Li, Xueri ^{[2
]}

Yin, Xiangping ^{[2
]}

Li, Baoyu ^{[2
]}

Han, Xiaorong ^{[2
]}

Li, Yanmin ^{[1
,2
]}

Zhang, Zhidong ^{[1
,2
]}

Liu, Jixing ^{[2
]}

机构：

[1] Inst Anim Hlth, Woking GU21 2RJ, Surrey, England

[2] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Minist Agr,Key Lab Anim Virol, Lanzhou 730046, Gansu, Peoples R China

来源：

VACCINE | 2008年 / 26卷 / 43期

关键词：

Bi-functional vaccine; DNA vaccine; Antisense RNA; Foot-and-mouth disease virus;

D O I：

10.1016/j.vaccine.2008.07.060

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To overcome the inability of a conventional vaccine against a virus to induce rapid protection against vital challenge, a novel strategy was performed to generate a novel bi-functional vector expressing antisense RNA targeted to 5 untranslated Legions (UTR) and VP1 protein of foot-and-mouth disease virus (FMDV). FMDV 5'UTR containing the vital RNA replication start elements was inserted inversely into the pIRES vector to produce antisense RNA, followed by insertion of FMDV VP1 gene to generate a recombinant plasmid pAS-IR-VP1. BHK-21 cells transfected with pAS-IR-VP1 plasmid showed a specific resistance against FMDV infection. In mice vaccinated with this plasmid, T cell proliferation was significantly higher than that in all unvaccinated control group. Anti-FMDV antibodies were detected LIP to 1 :64 in the serum collected from mice boosted with pAS-IR-VP1 at 21 clays after the first immunization. At 6 It post-vaccination 50-83% of the suckling mice survived a challenge with FMDV. The results demonstrated that a novel bi-functional DNA vaccine, producing antisense RNA targeted to FMDV 5'UTR and expressing VP1 protein, has been successfully Constructed and was able to induce a rapid inhibitory effect and immune response against FMDV infection in mice. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：5477 / 5483

页数：7