Comparison of ATP Level in U937 Cells in Vitro Using Two Different Photosensitizers in Photodynamic Therapy

被引：1

作者：

Nakajima, Nobue ^{[1
]}

Kawashima, Norimichi ^{[1
]}

机构：

[1] Toin Univ Yokohama, Ctr Biomed Engn, Aoba Ku, Yokohama, Kanagawa 2258502, Japan

来源：

12TH WORLD CONGRESS OF THE INTERNATIONAL PHOTODYNAMIC ASSOCIATION: PHOTODYNAMIC THERAPY: BACK TO THE FUTURE | 2009年 / 7380卷

关键词：

ATP level; photodynamic therapy; photosensitizer; 5-aminolevulinic acid; Hypericin; 5-AMINOLEVULINIC ACID; PROTOPORPHYRIN-IX; GLIOMA-CELLS; HYPERICIN; APOPTOSIS; CANCER; LIGHT;

D O I：

10.1117/12.822663

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The effect of PDT on ATP level in the Leukemic monocyte lymphoma cell line (U937) was studied using two different photosensitizers, PpIX and Hypericin. U-937 cells were irradiated (with and without ALA) and stored at room temperature. An LED lamp with a maximum peak of 637nm was used for ALA-PDT as a light source. A Na-Li lamp with a wavelength region of 560-730nm was used for Hypericin-PDT as a light source. We measured cell viability, the number of viable cells and ATP luminescence. As a result, it is evident that the irradiation time in the most effective PDT is equal to the time of the highest ATP level in the cells in ALA-PDT. This study suggests that it is possible to obtain suitable PDT conditions inducing apoptosis by measuring the ATP level in ALA-PDT. On the other hand, ATP level in Hypericin-PDT was increased with the decreasing in the cell viability. Hypericin-PDT induced apoptosis just after irradiation.

引用

页数：10

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