Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil

被引：37

作者：

Freitas, Ronaldo B. ^{[1
]}

Melo, Fernando L. ^{[2
]}

Oliveira, Darleise S. ^{[1
]}

Romano, Camila M. ^{[2
]}

Freitas, Maria Rute C. ^{[1
]}

Macedo, Olinda ^{[1
]}

Linhares, Alexandre C. ^{[1
]}

Zanotto, Paolo M. de A. ^{[2
]}

Durigon, Edison Luiz ^{[3
]}

机构：

[1] Minist Saude, Secretaria Vigilancia Saude, Inst Evandro Chagas, Secao Virol, BR-67037000 Ananindeua, Para, Brazil

[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Evolucao Mol & Bioinformac, BR-05508900 Sao Paulo, Brazil

[3] Univ Sao Paulo, Inst Ciencias Biomed ICB 2, Dept Microbiol, Lab Virol Clin, BR-05508900 Sao Paulo, Brazil

来源：

JOURNAL OF CLINICAL VIROLOGY | 2008年 / 43卷 / 01期

关键词：

erythrovirus B19; genotypes; 1; and; 3; clinical manifestations; phylogenetic analysis;

D O I：

10.1016/j.jcv.2008.03.033

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Human erythrovirus B 19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations. Objectives: To assess the prevalence of erythrovirus B 19 infection and the relative frequency of erythrovirus B 19 genotypes in patients in the Amazon region with various clinical presentations. Study design: A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested using specific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction. Results: B 19 DNA was detected in 117 (24%) of 487 samples. Of these, 106 (91%) isolates were genotype I and II (9%) were genotype 3. No genotype 2 was found. Genotype I had three clusters (A1, A2 and 13) and all genotype 3 sequences were subtype 3b. All patients with hernatological disorders within cluster B of genotype I were infected by the sarne B 19 lineage, suggesting that this lineage of B 19 may have been transmitted via transfusion of blood products. Conclusion: We reported two genotypes, I and 3b, with three genotype I clusters co-circulating in the Amazon region during the past 10 years. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：60 / 65

页数：6

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