Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures

被引：3

作者：

Hernandez, Mateo R. ^{[1
]}

Towns, Elyse N. ^{[1
]}

Ng, Terry C. ^{[1
]}

Walsh, Brian C. ^{[1
]}

Osibanjo, Richard ^{[1
]}

Parikh, Atul N. ^{[2
,3
]}

Land, Donald P. ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

[2] Univ Calif Davis, Dept Biomed Engn, Davis, CA 95616 USA

[3] Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA

来源：

APPLIED OPTICS | 2012年 / 51卷 / 15期

关键词：

QUARTZ-CRYSTAL MICROBALANCE; PHOSPHOLIPID MONOLAYERS; LIPOSOME ADSORPTION; VESICLE ADSORPTION; BILAYER FORMATION; SURFACE; MEMBRANES; INTERFACE;

D O I：

10.1364/AO.51.002842

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Attenuated total reflectance Fourier transform infrared spectroscopy is used to monitor the adsorption of 100 nm 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles to the surfaces of Ge, electrolessly deposited Au, and a well formed self-assembled monolayer of 1-octadecanethiol. The interaction of DPPC vesicles in solution with these different surfaces yields distinctly different surface structures: intact DPPC vesicles on Ge, a supported phospholipid bilayer on an electrolessly deposited Au surface, and a phospholipid monolayer onto the hydrophobic self-assembled monolayer. IR peak position, bandwidth, and intensity are used to confirm structure formation and quantitation of the amount of lipid that desorbs during film formation. (C) 2012 Optical Society of America

引用

页码：2842 / 2846

页数：5

共 21 条

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