Protocol Protocol for the setup and use of a human choroid plexus endothelial-epithelial two-cell-type in vitro model

被引：0

作者：

Herold, Rosanna ^{[1
,2
]}

Borkowski, Julia ^{[1
,2
]}

Schwerk, Christian ^{[1
,2
]}

Stump-Guithier, Carolin ^{[1
,2
]}

Lampe, Marko ^{[3
]}

Ishikawa, Hiroshi ^{[4
]}

Muranyi, Walter ^{[1
,2
]}

Schroten, Horst ^{[1
,2
]}

机构：

[1] Heidelberg Univ, Med Fac Mannheim, Dept Pediat, Pediat Infect Dis, D-68167 Mannheim, Germany

[2] Heidelberg Univ, Med Fac Mannheim, European Ctr Angiosci, D-68167 Mannheim, Germany

[3] European Mol Biol Lab, Adv Light Microscopy Core Facil, D-69117 Heidelberg, Germany

[4] Univ Tsukuba, Fac Med, Dept Neurosurg, Lab Clin Regenerat Med, Tennodai 1-1-1, Tsukuba, Ibaraki 3058575, Japan

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 04期

关键词：

Cell biology; Cell culture; Molecular biology; Neuroscience;

D O I：

10.1016/j.xpro.2022.101816

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Choroid plexus, located in brain ventricles, is the site of blood-cerebrospinal fluid barrier that contains endothelial cells and an epithelial monolayer separated by stroma. We established a two-cell-type model of the human choroid plexus con-sisting of immortalized endothelial cells (iHCPEnC) and epithelial papilloma (HIBCPP) cells grown on opposite sides of filter supports. In this protocol, we describe the preparation of this model, the measurement of transepithelial elec-trical resistance (TEER), and immunofluorescence imaging-based analysis to determine the barrier function. For complete details on the use and execution of this protocol, please refer to Muranyi et al. (2022).

引用

页数：12

共 5 条

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