Safe CRISPR: Challenges and Possible Solutions

被引：48

作者：

Pineda, Michael ^{[1
]}

Lear, Ashley ^{[1
]}

Collins, James P. ^{[2
]}

Kiani, Samira ^{[1
,3
]}

机构：

[1] Arizona State Univ, Sch Biol & Hlth Syst Engn, Tempe, AZ 85287 USA

[2] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA

[3] Www Kianilab Com, Tempe, AZ 85281 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2019年 / 37卷 / 04期

关键词：

GENE-DRIVE; SELFISH GENES; SYSTEM; DELIVERY; VECTOR; NANOPARTICLES; TECHNOLOGIES; SPECIFICITY; NUCLEASES; EVOLUTION;

D O I：

10.1016/j.tibtech.2018.09.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Applications of CRISPR in human health and in gene drives are at the forefront of biological research as tools. This technology will affect humankind and our environment, so as this technology pushes forward, the design and implementation of safety measures is imperative. Novel technologies and forethought in various applications of CRISPR are essential for using this technology safely. Here, we review environmental and health-related safety concerns associated with using CRISPR and ways proposed to minimize risk.

引用

页码：389 / 401

页数：13

共 98 条

[91] In vivo gene therapy potentials of CRISPR-Cas9 [J].

Xue, H-Y ;

Zhang, X. ;

Wang, Y. ;

Xiaojie, L. ;

Dai, W-J ;

Xu, Y. .

GENE THERAPY, 2016, 23 (07) :557-559

[92] A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice [J].

Yang, Yang ;

Wang, Lili ;

Bell, Peter ;

McMenamin, Deirdre ;

He, Zhenning ;

White, John ;

Yu, Hongwei ;

Xu, Chenyu ;

Morizono, Hiroki ;

Musunuru, Kiran ;

Batshaw, Mark L. ;

Wilson, James M. .

NATURE BIOTECHNOLOGY, 2016, 34 (03) :334-+

[93] In vivo base editing of post-mitotic sensory cells [J].

Yeh, Wei-Hsi ;

Chiang, Hao ;

Rees, Holly A. ;

Edge, Albert S. B. ;

Liu, David R. .

NATURE COMMUNICATIONS, 2018, 9

[94] Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo [J].

Yin, Hao ;

Song, Chun-Qing ;

Dorkin, Joseph R. ;

Zhu, Lihua J. ;

Li, Yingxiang ;

Wu, Qiongqiong ;

Park, Angela ;

Yang, Junghoon ;

Suresh, Sneha ;

Bizhanova, Aizhan ;

Gupta, Ankit ;

Bolukbasi, Mehmet F. ;

Walsh, Stephen ;

Bogorad, Roman L. ;

Gao, Guangping ;

Weng, Zhiping ;

Dong, Yizhou ;

Koteliansky, Victor ;

Wolfe, Scot A. ;

Langer, Robert ;

Xue, Wen ;

Anderson, Daniel G. .

NATURE BIOTECHNOLOGY, 2016, 34 (03) :328-+

[95] Non-viral vectors for gene-based therapy [J].

Yin, Hao ;

Kanasty, Rosemary L. ;

Eltoukhy, Ahmed A. ;

Vegas, Arturo J. ;

Dorkin, J. Robert ;

Anderson, Daniel G. .

NATURE REVIEWS GENETICS, 2014, 15 (08) :541-555

[96] Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype [J].

Yin, Hao ;

Xue, Wen ;

Chen, Sidi ;

Bogorad, Roman L. ;

Benedetti, Eric ;

Grompe, Markus ;

Koteliansky, Victor ;

Sharp, Phillip A. ;

Jacks, Tyler ;

Anderson, Daniel G. .

NATURE BIOTECHNOLOGY, 2014, 32 (06) :551-553

[97] In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice [J].

Zhou, Haibo ;

Liu, Junlai ;

Zhou, Changyang ;

Gao, Ni ;

Rao, Zhiping ;

Li, He ;

Hu, Xinde ;

Li, Changlin ;

Yao, Xuan ;

Shen, Xiaowen ;

Sun, Yidi ;

Wei, Yu ;

Liu, Fei ;

Ying, Wenqin ;

Zhang, Junming ;

Tang, Cheng ;

Zhang, Xu ;

Xu, Huatai ;

Shi, Linyu ;

Cheng, Leping ;

Huang, Pengyu ;

Yang, Hui .

NATURE NEUROSCIENCE, 2018, 21 (03) :440-+

[98] Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo [J].

Zuris, John A. ;

Thompson, David B. ;

Shu, Yilai ;

Guilinger, John P. ;

Bessen, Jeffrey L. ;

Hu, Johnny H. ;

Maeder, Morgan L. ;

Joung, J. Keith ;

Chen, Zheng-Yi ;

Liu, David R. .

NATURE BIOTECHNOLOGY, 2015, 33 (01) :73-80

← 1 2 3 4 5 6 7 8 9 10 →