Molecular detection of toxoplasma gondii DNA in goats (Capra hircus), sheep (Ovis aries), and donkey (Equus asinus) milk using PCR in East Azerbaijan province, Iran

被引：7
作者：
Deljavan, Naeimeh [1 ]
Moosavy, Mir-Hassan [1 ]
Hajipour, Nasser [1 ,2 ,3 ]
机构：
[1] Univ Tabriz, Fac Vet Med, Dept Food Hyg & Aquat, Tabriz, Iran
[2] Univ Tabriz, Fac Vet Med, Dept Pathobiol, Tabriz, Iran
[3] Shohadaye Ghavvas Blvd,Opposite Khavaran Town, Tabriz, East Azarbaijan, Iran
来源：
RESEARCH IN VETERINARY SCIENCE | 2022年 / 152卷
关键词：
Toxoplasma gondii; Milk; Sheep; Goat; Donkey; PCR; Iran; SEROPREVALENCE; ANTIBODIES; QUALITY;
D O I：
10.1016/j.rvsc.2022.07.020
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Toxoplasmosis, a zoonotic infection that is significant for public health (immunocompromised patients, pregnant women) and veterinary medicine (economic losses in the herd), is caused by an intracellular protozoan parasite belonging to the phylum Apicomplexa called Toxoplasma gondii. Consumption of unpasteurized milk and contaminated undercooked meat is a significant source for humans. The present study aimed to determine Toxoplasma gondii DNA in sheep, goats and donkeys Milk kept in East Azerbaijan province using the PCR method based on the B1 gene. For this purpose, 100 milk samples, including 45 sheep, 45 goats and 10 donkeys, were collected from different regions of northwestern Iran using direct milking and then transferred to the Food and Aquatic Health Laboratory under refrigerated conditions. The results showed that out of 100 milk samples examined, 16 samples (16%) were contaminated, and Toxoplasma gondii DNA was detected in 5 (11.11%) sheep, 9 (20%) goats and 2 (20%) donkeys milk specimen, respectively. These findings indicated that Toxoplasma gondii contaminated the raw milk, a human infection source.
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收藏
页码：58 / 60
页数：3
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