The first ratiometric fluorescent probe for aminopeptidase N

被引：27

作者：

Chen, Laizhong ^{[1
]}

Sun, Wei ^{[1
]}

Li, Wenhua ^{[1
]}

Li, Jing ^{[1
]}

Du, Lupei ^{[1
]}

Xu, Wenfang ^{[1
]}

Fang, Hao ^{[1
]}

Li, Minyong ^{[1
]}

机构：

[1] Shandong Univ, Sch Pharm, Dept Med Chem, Key Lab Chem Biol Nat Prod MOE, Jinan 250012, Shandong, Peoples R China

来源：

ANALYTICAL METHODS | 2012年 / 4卷 / 09期

关键词：

IN-VIVO; CELLS; INHIBITORS; TARGET; APN/CD13; DESIGN;

D O I：

10.1039/c2ay25556a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The current work describes the design and synthesis of the first ratiometric fluorescent probe, Ala-PABA-7HC, for the detection of aminopeptidase N (APN) at both the enzymatic and the cellular level. Upon addition of APN to the solution of Ala-PABA-7HC in PBS or culture medium, the maximum emission peak experienced a red shift from 390 nm to 450 nm, and the ratio of fluorescence intensity (I-450/I-390) (lambda(ex) = 330 nm) significantly increased from 0.26 to 3.05 (R = 11.7-fold) in 15 min.

引用

收藏

页码：2661 / 2663

页数：3

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