Investigation of dense fine speckled pattern and anti-dense fine speckled 70 antibody by a single step assay

被引：0

作者：

Onarer, Pelin ^{[1
,2
,3
]}

Mutlu, Esvet ^{[1
,4
]}

Ongut, Gozde ^{[1
,5
]}

Gultekin, Meral ^{[1
,5
]}

机构：

[1] Akdeniz Univ, Dept Med Microbiol, Fac Med, Antalya, Turkey

[2] Amasya Sabuncuoglu Serefeddin Training & Res Hosp, Amasya, Turkey

[3] Amasya Sabuncuoglu Serefeddin Egitim Arastirma Ha, Tibbi Mikrobiyol ABD, TR-05200 Amasya, Turkey

[4] Akdeniz Univ, Tip Fak, Tibbi Mikrobiyol ABD, Dekanl Binasi B Blok Kat 4, TR-07070 Antalya, Turkey

[5] Akdeniz Univ, Tip Fak, Tibbi Mikrobiyol ABD, B Blok Kat 2, TR-07070 Antalya, Turkey

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2022年 / 203卷

关键词：

Anti-DFS70; Dense fine speckled pattern; Indirect immunofluorescence method; ANTI-DFS70; ANTIBODIES; HEP-2; CELLS; ANTINUCLEAR AUTOANTIBODIES; HEALTHY-INDIVIDUALS;

D O I：

10.1016/j.mimet.2022.106606

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The indirect immunofluorescence (IIF) method is the gold standard for identifying anti-nuclear antibodies (ANAs). It is recommended that ANA, including the dense fine speckled (DFS) pattern, should be verified with a highly specific confirmatory test after a sensitive screening test. Although methods such as ELISA and LIA are often used to confirm the presence of anti-DFS70 antibodies, new IIF methods have been developed in recent years to prevent the difficulties in the recognition of the DFS pattern and to carry out the confirmatory test in a single step. In this study, we evaluated CytoBead (Generic Assays, Germany) test, which contained both HEp-2 cell substrate and beads coated with DFS70 antigen in one well, in comparison to the routine two-step test strategy. Five hundred forty-one samples were studied by conventional IIF assay, LIA, and CytoBead assay; 264 samples were studied by ELISA. The Bead component of the CytoBead test was found to be reliable as a confirmational test when compared with ELISA and LIA (total agreement values were 85.6% and 87.6%, respectively). The CytoBead ANA DFS70 might be a promising test in the future, allowing both screening and confirmation in a single step, saving time and being easier than two-step testing.

引用

页数：5

共 30 条

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