A quantitative bioapatite/collagen calibration method using Raman spectroscopy of bone

被引：27

作者：

Karampas, Ioannis A. ^{[1
,2
]}

Orkoula, Malvina G. ^{[1
,2
]}

Kontoyannis, Christos G. ^{[1
,2
]}

机构：

[1] Univ Patras, Dept Pharm, Patras 26500, Greece

[2] FORTH, Inst Chem Engn & High Temp Chem Proc, Patras, Greece

来源：

JOURNAL OF BIOPHOTONICS | 2013年 / 6卷 / 08期

关键词：

collagen; bone mineral; proline; amide I; hydroxyproline; quantitative analysis; bioapatite; Raman spectroscopy; COLLAGEN CROSS-LINKS; AGE; TISSUE; STRENGTH; SPECTRUM; QUALITY;

D O I：

10.1002/jbio.201200053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerous calibration models were developed and tested for the quantitative analysis of collagen and bioapatite in bone using Raman spectroscopy. (1) phosphate vibration at 960 cm(-1) was used as indicator of the content of bioapatite while for collagen three markers were used: the C-H-2 band at 2940 cm(-1), the amide I band at 1667 cm(-1) and the vibrations of proline and hydroxyproline at 855 and 878 cm(-1), respectively. Also a calibration model based on the PLS algorithm was developed, too. Validation of the derived calibration models indicated that the model that makes use of the height ratio of the peaks 960/(855+878) exhibits the best accuracy. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

引用

页码：573 / 586

页数：14

共 54 条

[1] On the increasing fragility of human teeth with age: A deep-UV resonance Raman study [J].

Ager, Joel W., III ;

Nalla, Ravi K. ;

Balooch, Guive ;

Kim, Grace ;

Pugach, Megan ;

Habelitz, Stefan ;

Marshall, Grayson W. ;

Kinney, John H. ;

Ritchie, Robert O. .

JOURNAL OF BONE AND MINERAL RESEARCH, 2006, 21 (12) :1879-1887

[2] Deep-ultraviolet Raman spectroscopy study of the effect of aging on human cortical bone [J].

Ager, JW ;

Nalla, RK ;

Breeden, KL ;

Ritchie, RO .

JOURNAL OF BIOMEDICAL OPTICS, 2005, 10 (03)

[3] Aging of microstructural compartments in human compact bone [J].

Akkus, O ;

Polyakova-Akkus, A ;

Adar, F ;

Schaffler, MB .

JOURNAL OF BONE AND MINERAL RESEARCH, 2003, 18 (06) :1012-1019

[4]

Arguello-Guerra V., 2009, BONE PATHOLOGY, P245

[5] Carbonate assignment and calibration in the raman spectrum of apatite [J].

Awonusi, Ayorinde ;

Morris, Michael D. ;

Tecklenburg, Mary M. J. .

CALCIFIED TISSUE INTERNATIONAL, 2007, 81 (01) :46-52

[6] STANDARD NORMAL VARIATE TRANSFORMATION AND DE-TRENDING OF NEAR-INFRARED DIFFUSE REFLECTANCE SPECTRA [J].

BARNES, RJ ;

DHANOA, MS ;

LISTER, SJ .

APPLIED SPECTROSCOPY, 1989, 43 (05) :772-777

[7] Good preservation of initial mechanical properties in lipid-extracted, disinfected, freeze-dried sheep patellar tendon grafts [J].

Bettin, D ;

Polster, J ;

Rullkötter, V ;

von Versen, R ;

Fuchs, S .

ACTA ORTHOPAEDICA SCANDINAVICA, 2003, 74 (04) :470-475

[8] Chemical and structural characterization of the mineral phase from cortical and trabecular bone [J].

Bigi, A ;

Cojazzi, G ;

Panzavolta, S ;

Ripamonti, A ;

Roveri, N ;

Romanello, M ;

Suarez, KN ;

Moro, L .

JOURNAL OF INORGANIC BIOCHEMISTRY, 1997, 68 (01) :45-51

[9] Comparison of mineral quality and quantity in iliac crest biopsies from high- and low-turnover osteoporosis: an FT-IR microspectroscopic investigation [J].

Boskey, AL ;

DiCarlo, E ;

Paschalis, E ;

West, P ;

Mendelsohn, R .

OSTEOPOROSIS INTERNATIONAL, 2005, 16 (12) :2031-2038

[10] Raman imaging demonstrates FGF2-induced craniosynostosis in mouse calvaria [J].

Crane, NJ ;

Morris, MD ;

Ignelzi, MA ;

Yu, GG .

JOURNAL OF BIOMEDICAL OPTICS, 2005, 10 (03)

← 1 2 3 4 5 6 →