Selective staudinger modification of proteins containing p-azidophenylalanine

被引：82

作者：

Tsao, ML

Tian, F

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 12期

关键词：

amino acids; azides; conjugation; phage display; protein modifications;

D O I：

10.1002/cbic.200500314

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) The unnatural amino acid p-azidophenylalanine was genetically introduced into a phage-displayed peptide and mutant Z-domain protein. These azide-containing proteins were selectively and efficiently modified by using fluorescein-derived phosphines and the Staudinger ligation reaction (see picture). This work provides a powerful new tool for the selective modification of proteins with a variety of synthetic agents. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：2147 / 2149

页数：3

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