Determination of L-ascorbic acid in wines by direct injection liquid chromatography using a polymeric column

被引：29

作者：

Lopes, P ^{[1
]}

Drinkine, J ^{[1
]}

Saucier, C ^{[1
]}

Glories, Y ^{[1
]}

机构：

[1] Univ Victor Segalen Bordeaux 2, Fac Enol Bordeaux, UMR 1219, INRA, F-33405 Talence, France

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 555卷 / 02期

关键词：

liquid chromatography; L-ascorbic acid; wine;

D O I：

10.1016/j.aca.2005.09.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid method for the identification and quantification of L-ascorbic acid in wines by direct injection liquid chromatography equipped with a UV detection was developed. The levels of ascorbic acid were determined using a polymeric PLRP-S 100 A (5 mu m) column (150 mm x 4.6 mm) with a mobile water/trifluoroacetic acid (99/1, v/v) phase. The method is rapid (less than 5 min) and sensitive (LOQ of 5 mg L(-1)). The calibration curve of ascorbic acid was linear (r = 0.999) over a concentration range between 1 and 200 mg L(-1). Repeatability was less than 2.5% and the recovery over 95%. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：242 / 245

页数：4

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