Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery

被引：697
作者：
Godbey, WT
Wu, KK
Mikos, AG
机构：
[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA
[2] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77251 USA
[3] Univ Texas, Hlth Sci Ctr, Div Hematol, Houston, TX 77030 USA
[4] Univ Texas, Hlth Sci Ctr, Vasc Biol Res Ctr, Houston, TX 77030 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 09期
关键词：
D O I：
10.1073/pnas.96.9.5177
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Poly(ethylenimine) (PEI) is one of a number of polycations that has been used successfully to transfer genes into living cells. Although PEI shows promise in the field of gene therapy, to date no rigorous proof of mechanism has been published regarding the fate of PEI/DNA administered for transfection. Here we show, by using fluorescent labeling and confocal microscopy, the paths of PEI/DNA complexes from endocytosis to gene expression. We found that complexes attach to cell surfaces and migrate into clumps that are endocytosed. The endocytotic vesicles grow in number and size and are occasionally seen to lyse. Most interesting is the fact that endocytosed PEI, whether administered with or without DNA, undergoes nuclear localization in the form of ordered structures.
引用
收藏
页码：5177 / 5181
页数：5
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