Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics

被引：54

作者：

Duocastella, Marti ^{[1
]}

Sun, Bo ^{[1
]}

Arnold, Craig B. ^{[1
]}

机构：

[1] Princeton Univ, Dept Mech & Aerosp Engn, Princeton, NJ 08544 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 05期

关键词：

adaptive optics; cells; microfluidics; microscopy; real-time imaging; ACOUSTIC GRADIENT-INDEX; EXTENDED DEPTH; SECTIONING MICROSCOPY; 2-PHOTON MICROSCOPY; REFRACTION LENS; FIELD; ILLUMINATION; CELLS;

D O I：

10.1117/1.JBO.17.5.050505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.5.050505]

引用

页数：3

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