A Chemical Probe for Lysine Malonylation

被引：65
作者：
Bao, Xiucong [1 ]
Zhao, Qian [1 ]
Yang, Tangpo [1 ]
Fung, Yi Man Eva [1 ]
Li, Xiang David [1 ]
机构：
[1] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 18期
关键词：
click chemistry; lysine malonylation; metabolic labeling; posttranslational modifications; protein modifications; FLUORESCENT DETECTION; ACYLATION; REPORTERS; SIRT5;
D O I：
10.1002/anie.201300252
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Tag! You′re it! MalAM-yne is a chemical reporter for malonylation of lysines within proteins (see scheme), a newly identified posttranslational modification. MalAM-yne is cell-permeable and metabolically incorporated into proteins in living cells. Subsequent bioorthogonal tag conjugation allows the fluorescent visualization of cellular malonylation and profiling of malonylated proteins. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：4883 / 4886
页数：4
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