Mechanical characterization of HIV-1 with a solid-state nanopore sensor

被引：43

作者：

Darvish, Armin ^{[1
]}

Lee, Jung Soo ^{[2
]}

Peng, Bin ^{[2
]}

Saharia, Jugal ^{[2
]}

Sundaram, Ramalingam Venkat Kalyana ^{[1
,3
]}

Goyal, Gaurav ^{[4
]}

Bandara, Nuwan ^{[2
]}

Ahn, Chi Won ^{[5
]}

Kim, Jungsuk ^{[6
]}

Dutta, Prashanta ^{[7
]}

Chaiken, Irwin ^{[3
]}

Kim, Min Jun ^{[2
]}

机构：

[1] Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USA

[2] Southern Methodist Univ, Dept Mech Engn, Dallas, TX 75275 USA

[3] Drexel Univ, Coll Med, Dept Biochem & Mol Biol, Philadelphia, PA 19104 USA

[4] Quantum Biosyst Inc, Menlo Pk, CA USA

[5] Natl NanoFab Ctr, Nanomat Lab, Daejeon, South Korea

[6] Gachon Univ, Dept Biomed Engn, Incheon, South Korea

[7] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA

来源：

ELECTROPHORESIS | 2019年 / 40卷 / 05期

基金：

新加坡国家研究基金会; 美国国家科学基金会; 美国国家卫生研究院;

关键词：

Human immunodeficiency virus; Mechanical characterization; Resistive pulse; Solid-state nanopore; Viral maturity; ATOMIC-FORCE MICROSCOPY; LIPID-BILAYERS; SINGLE-PARTICLE; CHOLESTEROL; NANOPARTICLES; ORGANIZATION; DEFORMATION; RIGIDITY; CELLS; AFM;

D O I：

10.1002/elps.201800311

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Enveloped viruses fuse with cells to transfer their genetic materials and infect the host cell. Fusion requires deformation of both viral and cellular membranes. Since the rigidity of viral membrane is a key factor in their infectivity, studying the rigidity of viral particles is of great significance in understating viral infection. In this paper, a nanopore is used as a single molecule sensor to characterize the deformation of pseudo-type human immunodeficiency virus type 1 at sub-micron scale. Non-infective immature viruses were found to be more rigid than infective mature viruses. In addition, the effects of cholesterol and membrane proteins on the mechanical properties of mature viruses were investigated by chemically modifying the membranes. Furthermore, the deformability of single virus particles was analyzed through a recapturing technique, where the same virus was analyzed twice. The findings demonstrate the ability of nanopore resistive pulse sensing to characterize the deformation of a single virus as opposed to average ensemble measurements.

引用

页码：776 / 783

页数：8

共 46 条

[1] Observations of Tunable Resistive Pulse Sensing for Exosome Analysis: Improving System Sensitivity and Stability [J].