Synthesis and evaluation of a novel fluorescent chemosensor for glutathione based on a rhodamine B and N-[4-(carbonyl) phenyl] maleimide conjugate and its application in living cell imaging

被引：27
|
作者：
Shu, Hai [1 ]
Wu, Xiaolei [1 ]
Zhou, Baojing [3 ]
Han, Yingbin [3 ]
Jin, Mingjie [1 ]
Zhu, Jing [2 ]
Bao, Xiaofeng [1 ]
机构：
[1] Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, 200 Xiaolinwei, Nanjing 210094, Jiangsu, Peoples R China
[2] Nanjing Univ Chinese Med, Dept Pharm, 138 XianlinDadao, Nanjing Shi 210023, Jiangsu Sheng, Peoples R China
[3] Nanjing Univ Sci & Technol, Sch Chem Engn, Nanjing 210094, Jiangsu, Peoples R China
来源：
DYES AND PIGMENTS | 2017年 / 136卷
基金：
高等学校博士学科点专项科研基金; 中国国家自然科学基金;
关键词：
Rhodamine B; Colorimetric fluorescent probe; GSH; Cell imaging; SENSOR;
D O I：
10.1016/j.dyepig.2016.08.063
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A novel rhodamine B spirolactam derivative bearing an N-[4-(carbonyl)phenyl] maleimide moiety (L1) was designed, synthesized and structurally characterized to develop a chemosensor. The interactions of L1 with amino acids and metal ions were studied by UV-vis absorption and fluorescence spectroscopy. L1 exhibited a highly sensitive and selective turn-on fluorescence response toward glutathione (GSH) over other biological species in EtOH/HEPES (3:2, v/v, 0.1 mM, pH 7.34) solution. The detection limit of GSH by L1 was 0.219 mu M. Intracellular imaging applications demonstrated that L1 can be used as a fluorescent probe for the detection of GSH in HepG-2 and HUVEC cells. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：535 / 542
页数：8
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