Rapid macroconidia production in Fusarium graminearum 3-and 15-acetyldeoxynivalenol (ADON) chemotypes using sucrose-water medium

被引：6

作者：

Ben Mansour, Manel ^{[1
,2
]}

Goh, Yit Kheng ^{[1
]}

Vujanovic, Vladimir ^{[1
]}

机构：

[1] Univ Saskatchewan, Dept Food & Bioprod Sci, Saskatoon, SK S7N 5A8, Canada

[2] Univ Tunis El Manar, Dept Plant Biotechnol, Tunis 1060, Tunisia

来源：

ANNALS OF MICROBIOLOGY | 2012年 / 62卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Fusarium graminearum; Chemotype; Macroconidia formation; HPLC; Mycotoxin; TRICHOTHECENE MYCOTOXINS; WHEAT; DEOXYNIVALENOL; RESISTANCE; BARLEY; GENE; POPULATIONS;

D O I：

10.1007/s13213-011-0335-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sucrose-water medium induced a high rate of macroconidia formation in Fusarium graminearum 3- and 15-acetyldeoxynivalenol (ADON) chemotypes. The F. graminearum isolates tested produced uniform macroconidia in large quantities and within a shorter incubation period in sucrose-water medium compared to other assessed media. At the same time, high rates of mycotoxin accumulation (trichothecenes: DON, 3-ADON, 15-ADON, and zearalenone: ZEA) in macroconidia were detected using high performance liquid chromatography. The proposed "saccharose-water" method represents a valuable alternative for conidia production in mycotoxigenic F. graminearum isolates, having advantages over the potentially mutagenic UV light, and potato-dextrose agar, which can degenerate macroconidia, and over expensive chemical compounds or natural substances for which the preparation protocols and handling procedures are relatively complex or time-consuming.

引用

页码：965 / 971

页数：7

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