Structures from Anomalous Diffraction of Native Biological Macromolecules

被引:134
作者
Liu, Qun [1 ]
Dahmane, Tassadite [2 ]
Zhang, Zhen [2 ]
Assur, Zahra [2 ]
Brasch, Julia [2 ]
Shapiro, Lawrence [2 ]
Mancia, Filippo [3 ]
Hendrickson, Wayne A. [1 ,2 ,3 ,4 ]
机构
[1] Brookhaven Natl Lab, Natl Synchrotron Light Source NSLS X4, New York Struct Biol Ctr, Upton, NY 11973 USA
[2] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA
[3] Columbia Univ, Dept Physiol & Cellular Biophys, New York, NY 10032 USA
[4] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA
关键词
SOFT X-RAYS; CRYSTAL-STRUCTURE; RADIATION-DAMAGE; SULFUR; SIGNAL; RESOLUTION; CRYSTALLOGRAPHY; REDUNDANCY; MAD;
D O I
10.1126/science.1218753
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Crystal structure analyses for biological macromolecules without known structural relatives entail solving the crystallographic phase problem. Typical de novo phase evaluations depend on incorporating heavier atoms than those found natively; most commonly, multi- or single-wavelength anomalous diffraction (MAD or SAD) experiments exploit selenomethionyl proteins. Here, we realize routine structure determination using intrinsic anomalous scattering from native macromolecules. We devised robust procedures for enhancing the signal-to-noise ratio in the slight anomalous scattering from generic native structures by combining data measured from multiple crystals at lower-than-usual x-ray energy. Using this multicrystal SAD method (5 to 13 equivalent crystals), we determined structures at modest resolution (2.8 to 2.3 angstroms) for native proteins varying in size (127 to 1148 unique residues) and number of sulfur sites (3 to 28). With no requirement for heavy-atom incorporation, such experiments provide an attractive alternative to selenomethionyl SAD experiments.
引用
收藏
页码:1033 / 1037
页数:5
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