Imaging of bioluminescent LNCaP-luc-M6 tumors: A new animal model for the study of metastatic human prostate cancer

被引：56

作者：

Scatena, CD ^{[1
]}

Hepner, MA ^{[1
]}

Oei, YA ^{[1
]}

Dusich, JM ^{[1
]}

Yu, SF ^{[1
]}

Purchio, T ^{[1
]}

Contag, PR ^{[1
]}

Jenkins, DE ^{[1
]}

机构：

[1] Xenogen Corp, Alameda, CA 94501 USA

来源：

PROSTATE | 2004年 / 59卷 / 03期

关键词：

orthotopic prostate model; biophotonic imaging (BLI); firefly luciferase; mouse xenograft model; micrometastasis;

D O I：

10.1002/pros.20003

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

BACKGROUND. Animal experiments examining hormone-sensitive metastatic prostate cancer using the human LNCaP cell line have been limited to endpoint analyses. To permit longitudinal studies, we generated a luciferase-expressing cell line and used bioluminescent imaging (BLI) to non-invasively monitor the in vivo growth of primary LNCaP tumors and metastasis. METHODS. LNCaP.FGC cells were transfected to constitutively express firefly luciferase. LNCaP-luc-M6 cells were tested for bioluminescent signal intensity and hormone responsiveness in vitro. The cells were implanted in subcutaneous and orthotopic sites in SCID-bg mice and imaged over time. RESULTS. The LNCaP-luc-M6 cells formed subcutaneous and orthotopic tumors in SCID-bg mice, and nearly all tumor-bearing animals developed pulmonary metastases. Early detection and temporal growth of primary tumors and metastatic lesions was successfully monitored by BLI. CONCLUSIONS. The LNCaP-luc-M6 cell line is a bioluminescent, hormone-sensitive prostate cancer cell line applicable for BLI studies to non-invasively monitor subcutaneous and orthotopic prostate tumor growth and metastasis in vivo. (C) 2004 Wiley-Liss, Inc.

引用

页码：292 / 303

页数：12

共 41 条

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