Interaction of the UV-damaged DNA-binding protein with hepatitis B virus X protein is conserved among mammalian hepadnaviruses and restricted to transactivation-proficient X-insertion mutants

被引：99
作者：
Sitterlin, D
Lee, TH
Prigent, S
Tiollais, P
Butel, JS
Transy, C
机构：
[1] INST PASTEUR,UNITE RECOMBINAT & EXPRESS GENET,INSERM,U163,F-75724 PARIS 15,FRANCE
[2] BAYLOR COLL MED,DIV MOL VIROL,HOUSTON,TX 77030
来源：
JOURNAL OF VIROLOGY | 1997年 / 71卷 / 08期
关键词：
D O I：
10.1128/JVI.71.8.6194-6199.1997
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We carried out a comparative analysis of several proposed host protein partners of the human hepatitis B virus X protein (HBx) using both the GAL-4- and the LexA-based yeast two-hybrid system. We showed that the interaction of HBx with the UV-damaged DNA-binding protein (UVDDB) is positive in both yeast systems, detectable in cotransfected human cells, conserved by rodent hepadnavirus X proteins (known to transactivate in human cells), and tightly correlated with the transactivation proficiency of X-insertion mutants. Taken together, our results strongly suggest that UVDDB is involved in X-mediated transactivation.
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收藏
页码：6194 / 6199
页数：6
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