Vaspin Attenuates RANKL-Induced Osteoclast Formation in RAW264.7 Cells

被引：42

作者：

Kamio, Noriaki ^{[1
,2
]}

Kawato, Takayuki ^{[3
,4
]}

Tanabe, Natsuko ^{[3
,4
]}

Kitami, Satoshi ^{[3
]}

Morita, Toyoko ^{[3
,5
]}

Ochiai, Kuniyasu ^{[1
,2
]}

Maeno, Masao ^{[3
,4
]}

机构：

[1] Nihon Univ, Sch Dent, Dept Microbiol, Tokyo 1018310, Japan

[2] Nihon Univ, Sch Dent, Dent Res Ctr, Div Immunol & Pathol, Tokyo 1018310, Japan

[3] Nihon Univ, Sch Dent, Dept Oral Hlth Sci, Tokyo 1018310, Japan

[4] Nihon Univ, Sch Dent, Dent Res Ctr, Div Funct Morphol, Tokyo 1018310, Japan

[5] Lion Fdn Dent Hlth, Tokyo, Japan

来源：

CONNECTIVE TISSUE RESEARCH | 2013年 / 54卷 / 02期

关键词：

adipokine; osteoclastogenesis; NFATc1; MMP-9; cathepsin K; NF-KAPPA-B; GENE-EXPRESSION; RHEUMATOID-ARTHRITIS; ENDOTHELIAL-CELLS; ADIPOSE-TISSUE; CATHEPSIN-K; OBESITY; NFATC1; DIFFERENTIATION; RECEPTOR;

D O I：

10.3109/03008207.2012.761978

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Visceral adipose tissue-derived serine protease inhibitor (vaspin), an adipokine that was recently identified in a rat model of type 2 diabetes, has been suggested to have an insulin-sensitizing effect. In this study, we investigated whether vaspin inhibits receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis using two types of osteoclast precursors: RAW264.7 cells and bone marrow cells (BMCs). Vaspin inhibited RANKL-induced osteoclastogenesis in RAW264.7 cells and BMCs. Interestingly, vaspin also inhibited the RANKL-induced expression of nuclear factor of activated T cells c1 (NFATc1) in RAW264.7 cells and BMCs. Furthermore, it inhibited the RANKL-induced upregulation of matrix metalloproteinase-9 and cathepsin K in RAW264.7 cells. Thus, we suggest that vaspin downregulates osteoclastogenesis in part by inhibiting expression of the transcription factor NFATc1.

引用

页码：147 / 152

页数：6

共 29 条

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