Trypanosoma cruzi:: Effects of infection on cathepsin D activity in the midgut of Rhodnius prolixus

被引：43

作者：

Borges, EC ^{[1
]}

Machado, EMM ^{[1
]}

Garcia, ES ^{[1
]}

Azambuja, P ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, Lab Bioquim & Fisiol Insetos, BR-21045900 Rio De Janeiro, Brazil

来源：

EXPERIMENTAL PARASITOLOGY | 2006年 / 112卷 / 02期

关键词：

R; prolixus; Rhodnius prolixus; Triatominae; T; cruzi; Trypanosoma cruzi; Trypanosomatidae; cathepsin D (EC 3.4.23.5);

D O I：

10.1016/j.exppara.2005.09.008

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cathepsin D activity was estimated in midgut homogenates from Rhodnius profixus, uninfected and experimentally infected with Trypanosoma cruzi, at different times after blood ingestion. No enzyme activity was found in the anterior midgut and rectum. In the posterior midgut, enzyme activity was found both in lumen and wall. In starved uninfected insects, in lumen and wall, cathepsin D activity was high, decreasing to a constant rate at 1-15 days after feeding. In insects infected with T. cruzi cathepsin D activity increased I and 3 days after blood meal. We suggest that these changes in cathepsin D activity in R. prolixus posterior midgut are due to the establishment of T. cruzi infection. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：130 / 133

页数：4

共 19 条

[1] ACID AND NEUTRAL HYDROLASES IN TRYPANOSOMA-CRUZI - CHARACTERIZATION AND ASSAY [J].