miR-1289 and "Zipcode"-like Sequence Enrich mRNAs in Microvesicles

被引：225

作者：

Bolukbasi, Mehmet Fatih ^{[1
]}

Mizrak, Arda ^{[1
]}

Ozdener, Gokhan Baris ^{[1
]}

Madlener, Sibylle ^{[2
]}

Stroebel, Thomas ^{[3
]}

Erkan, Erdogan Pekcan ^{[2
]}

Fan, Jian-Bing ^{[4
]}

Breakefield, Xandra O. ^{[1
]}

Saydam, Okay ^{[2
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Neurol & Radiol, Boston, MA USA

[2] Med Univ Vienna, Dept Pediat, Div Oncol, Mol Neurooncol Res Unit, A-1090 Vienna, Austria

[3] Med Univ Vienna, Inst Neurol, A-1090 Vienna, Austria

[4] Illumina Inc, San Diego, CA USA

来源：

MOLECULAR THERAPY-NUCLEIC ACIDS | 2012年 / 1卷

关键词：

D O I：

10.1038/mtna.2011.2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Despite intensive studies, the molecular mechanisms by which the genetic materials are uploaded into microvesicles (MVs) are still unknown. This is the first study describing a zipcode-like 25 nucleotide (nt) sequence in the 3'-untranslated region (3'UTR) of mRNAs, with variants of this sequence present in many mRNAs enriched in MVs, as compared to their glioblastoma cells of origin. When this sequence was incorporated into the 3' UTR of a reporter message and expressed in a different cell type, it led to enrichment of the reporter mRNA in MVs. Critical features of this sequence are both a CUGCC core presented on a stemloop structure and a miRNA-binding site, with increased levels of the corresponding miRNA in cells further increasing levels of mRNAs in MVs.

引用

页数：10

共 45 条

[1] Intercellular transfer of the oncogenic receptor EGFrvIII by microvesicles derived from tumour cells
Al-Nedawi, Khalid
Meehan, Brian
Micallef, Johann
Lhotak, Vladimir
May, Linda
Guha, Abhijit
Rak, Janusz
[J]. NATURE CELL BIOLOGY, 2008, 10 (05) : 619 - U24
[2] Microvesicles Messengers and mediators of tumor progression
Al-Nedawi, Khalid
Meehan, Brian
Rak, Janusz
[J]. CELL CYCLE, 2009, 8 (13) : 2014 - 2018
[3] Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, Lydia
Seow, Yiqi
Yin, HaiFang
Betts, Corinne
Lakhal, Samira
Wood, Matthew J. A.
[J]. NATURE BIOTECHNOLOGY, 2011, 29 (04) : 341 - U179
[4] To localize or not to localize: mRNA fate is in 3′UTR ends
Andreassi, Catia
Riccio, Antonella
[J]. TRENDS IN CELL BIOLOGY, 2009, 19 (09) : 465 - 474
[5] INCREASED MIDKINE GENE-EXPRESSION IN HUMAN GASTROINTESTINAL CANCERS
ARIDOME, K
TSUTSUI, J
TAKAO, S
KADOMATSU, K
OZAWA, M
AIKOU, T
MURAMATSU, T
[J]. JAPANESE JOURNAL OF CANCER RESEARCH, 1995, 86 (07): : 655 - 661
[6] Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences
Balaj, Leonora
Lessard, Ryan
Dai, Lixin
Cho, Yoon-Jae
Pomeroy, Scott L.
Breakefield, Xandra O.
Skog, Johan
[J]. NATURE COMMUNICATIONS, 2011, 2
[7] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004)
Bartel, David P.
[J]. CELL, 2007, 131 (04) : 11 - 29
[8] Galanin and galanin receptors in human cancers
Berger, A
Santic, R
Hauser-Kronberger, C
Schilling, FH
Kogner, P
Ratschek, M
Gamper, A
Jones, N
Sperl, W
Kofler, B
[J]. NEUROPEPTIDES, 2005, 39 (03) : 353 - 359
[9] Origins and Mechanisms of miRNAs and siRNAs
Carthew, Richard W.
Sontheimer, Erik J.
[J]. CELL, 2009, 136 (04) : 642 - 655
[10] Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis
Cheng, AM
Byrom, MW
Shelton, J
Ford, LP
[J]. NUCLEIC ACIDS RESEARCH, 2005, 33 (04) : 1290 - 1297

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