Analysis of mechanisms of activation and repression at bacterial promoters

被引：20

作者：

Minchin, Stephen D. ^{[1
]}

Busby, Stephen J. W. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

METHODS | 2009年 / 47卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

Bacteria; Promoters; RNA polymerase; Regulation; Activators; Repressors; Genetics; Biochemistry; Bioinformatics; COLI-MELR PROMOTER; ESCHERICHIA-COLI; DNA-BINDING; TRANSCRIPTION ACTIVATION; PROTEIN; ORGANIZATION; MOLECULES; SEQUENCES; AFFINITY; COMPLEX;

D O I：

10.1016/j.ymeth.2008.10.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In bacteria, the expression of transcription units is controlled by regulatory regions, that contain one or more promoters and binding sites for regulatory proteins that activate or repress expression in response to different signals. In this chapter, we explain the diverse approaches that can be used to understand the mechanisms by which the different factors intervene, and how the effects are integrated. Bioinformatics, genetics and biochemistry must be combined to understand the organisation of regulatory regions and the mechanisms by which transcription initiation is controlled. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：6 / 12

页数：7

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