Genetic mapping of maize with the intermated MoV x KW7 population using MITE-AFLP and SSR markers

Molecular genetic markers have wide applicability for a various genetic analyses, and genetic mapping with PCR-based markers has identified many loci in the maize genome. This study was conducted to develop a genetic map of maize based on SSR and MITE-AFLP markers. The F-2 mapping population was established from a cross between Mo17 (American inbred line) and KW7 (Korean waxy corn landrace). Total of 198 markers, including 90 SSR and 108 MITE-AFLP markers, were segregated to map in the chromosomes in 128 F2 plants. Of these, 139 (70.2%) markers, including 66 MITE-AFLP and 73 SSR markers were found to be genetically linked to form 10 linkage groups. The size of framework map spanned 1,837.4 cM of the ten linkage groups. The average linkage distance between markers among all linkage groups was 13.2 cM. The number of markers per linkage group ranged from 6 to 22. The framework map developed in the present study mill he utilized for dissection of quantitative traits and identification of superior QTLs from the maize hybrid, and mill be used for the further genetic analysis and maize breeding programs.