Molecular Identification of the Yeast Strains Associated with Spontaneous Wine Fermentation of Kalecik Karasi and Emir Grapes

被引：0

作者：

Karasu-Yalcin, Seda ^{[1
]}

Senses-Ergul, Sule ^{[2
]}

Ozbas, Z. Yesim ^{[3
]}

机构：

[1] Abant Izzet Baysal Univ, Fac Engn & Architecture, Dept Food Engn, TR-14280 Golkoy, Bolu, Turkey

[2] Publ Hlth Inst Turkey, Dept Consumer Safety Labs, TR-06100 Ankara, Turkey

[3] Hacettepe Univ, Dept Food Engn, Fac Engn, TR-06532 Ankara, Turkey

来源：

JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2013年 / 7卷 / 04期

关键词：

Yeast; Wine; Spontaneous fermentation; Molecular identification; PCR-RFLP; RAPD-PCR; NON-SACCHAROMYCES YEASTS; DIFFERENTIATION; DYNAMICS; BIODIVERSITY; WINEMAKING; RDNA; RAPD; TOOL;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, 49 yeast isolates, which had been isolated during spontaneous wine fermentation of Kalecik Karasi and Emir grapes, were identified by molecular methods. Nineteen of the 24 Kalecik Karasi isolates and 22 of the 25 Emir isolates could be identified to species level by Restriction Fragment Length Polymorphism (RFLP) of the ITS-18S rRNA gene. The identified Kalecik Karasi isolates were found to be strains of Candida pulcherrima, Candida robusta, Cryptococcus albidus, Kloeckera apiculata, Kloeckera apis, Saccharomyces cerevisiae and Torulaspora delbrueckii. The Emir isolates belonged to Candida krusei, C. pulcherrima, K. apiculata and S. cerevisiae. All of the S. cerevisiae strains and most of the non-Saccharomyces strains could be differentiated by randomly amplified polymorphic DNA (RAPD-PCR) analysis. Although satisfactory results were obtained by M13 primer, (GTG)(3) primer was much more effective in the discrimination of K. apiculata, Cry. albidus and C. krusei strains.

引用

页码：2653 / 2672

页数：20

共 23 条

[1]

Boekhout T., 2003, YEAST FOOD BENEFICIA, P69, DOI DOI 10.1533/9781845698485.69

[2]

Bujdosó G, 2001, FOOD TECHNOL BIOTECH, V39, P19

[3] Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking [J].

Ciani, Maurizio ;

Comitini, Francesca ;

Mannazzu, Ilaria ;

Domizio, Paola .

FEMS YEAST RESEARCH, 2010, 10 (02) :123-133

[4] Molecular detection and identification of Brettanomyces/Dekkera bruxellensis and Brettanomyces/Dekkera anomalus in spoiled wines [J].

Cocolin, L ;

Rantsiou, K ;

Iacumin, L ;

Zironi, R ;

Comi, G .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (03) :1347-1355

[5]

Dequin S., 2003, YEASTS FOOD BENEFICI, P389

[6] Yeast dynamics during spontaneous wine fermentation of the Catalanesca grape [J].

Di Maro, Elena ;

Ercolini, Danilo ;

Coppola, Salvatore .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 117 (02) :201-210

[7] Restriction enzyme analysis of PCR amplified rDNA as a taxonomic tool in yeast identification [J].

Dlauchy, D ;

Tornai-Lehoczki, J ;

Péter, G .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1999, 22 (03) :445-453

[8]

Esteve-Zarzoso B, 1998, Int Microbiol, V1, P143

[9] Molecular identification and characterization of wine yeasts isolated from Tenerife (Canary Island, Spain) [J].

Gonzalez, S. S. ;

Barrio, E. ;

Querol, A. .

JOURNAL OF APPLIED MICROBIOLOGY, 2007, 102 (04) :1018-1025

[10] Rapid detection and quantification of yeast species during spontaneous wine fermentation by PCR-RFLP analysis of the rDNA ITS region [J].

Granchi, L ;

Bosco, M ;

Messini, A ;

Vincenzini, M .

JOURNAL OF APPLIED MICROBIOLOGY, 1999, 87 (06) :949-956

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