Effect of biomass adaptation to the degradation of anionic surfactants in laundry wastewater using EGSB reactors

被引:61
作者
Delforno, T. P. [1 ]
Moura, A. G. L. [1 ]
Okada, D. Y. [1 ]
Varesche, M. B. A. [1 ]
机构
[1] Univ Sao Paulo, EESC, LPB, Dept Hidraul & Saneamento, BR-13563120 Sao Carlos, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Linear alkylbenzene sulfonate (LAS); Expanded granular sludge bed (EGSB); Anaerobic reactor; Pyrosequencing; HYDRAULIC RETENTION TIME; ANAEROBIC-DIGESTION; UASB REACTORS; GEN; NOV; LAS; BIODEGRADATION; INHIBITION; SLUDGE; RNA; DIVERSITY;
D O I
10.1016/j.biortech.2013.11.102
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Two expanded granular sludge bed reactors were operated. R-AB (adapted biomass) was operated in two stages: Stage I, with standard LAS (13.2 mg L (1)); and Stage II, in which the standard LAS was replaced by diluted laundry wastewater according to the LAS concentration (11.2 mg L (1)). R-NAB (not adapted bio-mass) had a single stage, using direct wastewater (11.5 mg L (1)). Thus, the strategy of biomass adaptation did not lead to an increase of surfactant removal in wastewater (R-AB-Stage II: 77%; R-NAB-Stage I: 78%). By means of denaturing gradient gel electrophoresis, an 80% similarity was verified in the phases with laundry wastewater (sludge bed) despite the different reactor starting strategies. By pyrosequencing, many reads were related to genera of degraders of aromatic compounds and sulfate reducers (Syntrophorhabdus and Desulfobulbus). The insignificant difference in LAS removal between the two strategies was most likely due to the great microbial richness of the inoculum. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:114 / 121
页数:8
相关论文
共 36 条
[1]   SYNERGISTES-JONESII, GEN-NOV, SP-NOV - A RUMEN BACTERIUM THAT DEGRADES TOXIC PYRIDINEDIOLS [J].
ALLISON, MJ ;
MAYBERRY, WR ;
MCSWEENEY, CS ;
STAHL, DA .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1992, 15 (04) :522-529
[2]  
ALPHENAAR PA, 1993, BIORESOURCE TECHNOL, V43, P249, DOI 10.1016/0960-8524(93)90038-D
[3]   EFFECTS OF LIPIDS ON THERMOPHILIC ANAEROBIC-DIGESTION AND REDUCTION OF LIPID INHIBITION UPON ADDITION OF BENTONITE [J].
ANGELIDAKI, I ;
PETERSEN, SP ;
AHRING, BK .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 33 (04) :469-472
[4]   Anaerobic bioprocessing of sewage sludge, focusing on degradation of linear alkylbenzene sulfonates (LAS) [J].
Angelidaki, I ;
Toräng, L ;
Waul, CM ;
Schmidt, JE .
WATER SCIENCE AND TECHNOLOGY, 2004, 49 (10) :115-122
[5]  
Association A. P. H., 2005, STANDARD METHODS EXA, P521
[6]  
Braga J.K., 2011, 10 OF SEM LAT AM DIG
[7]  
Breznak JA, 2006, PROKARYOTES: A HANDBOOK ON THE BIOLOGY OF BACTERIA, VOL 4, THIRD EDITION, P991, DOI 10.1007/0-387-30744-3_34
[8]   The Ribosomal Database Project: improved alignments and new tools for rRNA analysis [J].
Cole, J. R. ;
Wang, Q. ;
Cardenas, E. ;
Fish, J. ;
Chai, B. ;
Farris, R. J. ;
Kulam-Syed-Mohideen, A. S. ;
McGarrell, D. M. ;
Marsh, T. ;
Garrity, G. M. ;
Tiedje, J. M. .
NUCLEIC ACIDS RESEARCH, 2009, 37 :D141-D145
[9]   Anaerobic degradation of linear alkylbenzene sulfonate (LAS) in fluidized bed reactor by microbial consortia in different support materials [J].
de Oliveira, Lorena Lima ;
Costa, Rachel Biancalana ;
Okada, Dagoberto Yukio ;
Vich, Daniele Vital ;
Silveira Duarte, Iolanda Cristina ;
Silva, Edson Luiz ;
Amancio Varesche, Maria Bernadete .
BIORESOURCE TECHNOLOGY, 2010, 101 (14) :5112-5122
[10]   Microbial characterization and removal of anionic surfactant in an expanded granular sludge bed reactor [J].
Delforno, T. P. ;
Okada, D. Y. ;
Polizel, J. ;
Sakamoto, I. K. ;
Varesche, M. B. A. .
BIORESOURCE TECHNOLOGY, 2012, 107 :103-109