Syncytin-1 and its receptor is present in human gametes

被引：36

作者：

Bjerregaard, B. ^{[1
]}

Lemmen, J. G. ^{[2
]}

Petersen, M. R. ^{[2
]}

Ostrup, E. ^{[1
]}

Iversen, L. H. ^{[2
]}

Almstrup, K. ^{[3
]}

Larsson, L. -I. ^{[1
]}

Ziebe, S. ^{[2
]}

机构：

[1] Univ Copenhagen, Fac Life Sci, Copenhagen, Denmark

[2] Rigshosp, Fertil Clin, Copenhagen Univ Hosp, DK-2100 Copenhagen, Denmark

[3] Rigshosp, Dept Growth & Reprod, Copenhagen Univ Hosp, DK-2100 Copenhagen, Denmark

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2014年 / 31卷 / 05期

关键词：

ASCT-2; Fertilization; Oocyte; Sperm; Syncytin; RETROVIRUS HERV-W; ENVELOPE GLYCOPROTEIN; SPERM; FUSION; PROTEINS; CELLS;

D O I：

10.1007/s10815-014-0224-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To determine whether the true fusogen Syncytin-1 and its receptor (ASCT-2) is present in human gametes using qRT-PCR, immunoblotting and immunofluorescence. Donated oocytes and spermatozoa, originating from a fertility center in tertiary referral university hospital, underwent qRT-PCR, immunoblotting and immunofluorescence analyzes. Quantitative RT-PCR of sperm samples from sperm donors showed that syncytin-1 is present in all samples, however, protein levels varied between donors. Syncytin-1 immunoreactivity predominates in the sperm head and around the equatorial segment. The receptor ASCT-2 is expressed in the acrosomal region and in the sperm tail. Moreover, ASCT-2, but not syncytin-1, is expressed in oocytes and the mRNA level increases with increasing maturity of the oocytes. Syncytin and its receptor are present in human gametes and localization and temporal appearance is consistent with a possible role in fusion between oocyte and sperm.

引用

页码：533 / 539

页数：7

共 31 条

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