Upregulation of smpd3 via BMP2 stimulation and Runx2

被引:21
作者
Chae, Young-Mi [1 ]
Heo, Sun-Hee [1 ]
Kim, Jae-Young [1 ]
Lee, Jae-Mok [2 ]
Ryoo, Hyun-Mo [3 ]
Cho, Je-Yoel [1 ]
机构
[1] Kyungpook Natl Univ, Sch Dent, Dept Biochem, Taegu, South Korea
[2] Kyungpook Natl Univ, Sch Dent, Dept Periodontol, Taegu, South Korea
[3] Seoul Natl Univ, Sch Dent, Dept Cell & Dev Biol, Seoul, South Korea
关键词
BMP2; Bone development; Runx2; Smpd3; BONE MORPHOGENETIC PROTEIN-2; NEUTRAL SPHINGOMYELINASE-2; EXPRESSION; CELLS; DIFFERENTIATION; DEGRADATION; OSTEOBLASTS; CERAMIDE; GROWTH; MOUSE;
D O I
10.5483/BMBRep.2009.42.2.086
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Deletion of smpd3 induces osteogenesis and dentinogenesis imperfecta in mice. smpd3 is highly elevated in the parietal bones of developing mouse calvaria, but not in sutural mesenchymes. Here, we examine the mechanism of smpd3 regulation, which involves BMP2 stimulation of Runx2. smpd3 mRNA expression increased in response to BMP2 treatment and Runx2 transfection in C2C12 cells. The Runx2-responsive element (RRE) encoded within the -562 to -557 region is important for activation of the smpd3 promoter by Runx2. Electrophoretic mobility shift assays revealed that Runx2 binds strongly to the -355 to -350 RRE and less strongly to the -562 to -557 site. Thus, the smpd3 promoter is activated by BMP2 and is directly regulated by the Runx2 transcription factor. This novel description of smpd3 regulation will aid further studies of bone development and osteogenesis. [BMB reports 2009; 42(2): 86-90]
引用
收藏
页码:86 / 90
页数:5
相关论文
共 24 条
  • [1] A deletion in the gene encoding sphingomyelin phosphodiesterase 3 (Smpd3) results in osteogenesis and dentinogenesis imperfecta in the mouse
    Aubin, I
    Adams, CP
    Opsahl, S
    Septier, D
    Bishop, CE
    Auge, N
    Salvayre, R
    Negre-Salvayre, A
    Goldberg, M
    Guénet, JL
    Poirier, C
    [J]. NATURE GENETICS, 2005, 37 (08) : 803 - 805
  • [2] Proteasomal degradation of Runx2 shortens parathyroid hormone-induced anti-apoptotic signaling in osteoblasts - A putative explanation for why intermittent administration is needed for bone anabolism
    Bellido, T
    Ali, AA
    Plotkin, LI
    Fu, Q
    Gubrij, I
    Roberson, PK
    Weinstein, RS
    O'Brien, CA
    Manolagas, SC
    Jilka, RL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (50) : 50259 - 50272
  • [3] Reciprocal temporospatial patterns of Msx2 and osteocalcin gene expression during murine odontogenesis
    Bidder, M
    Latifi, T
    Towler, DA
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1998, 13 (04) : 609 - 619
  • [4] Cho JY, 2006, GENE, V372, P71, DOI 10.1016/j.gene.2005.12.010
  • [5] Tumor-derived tumor necrosis factor-alpha promotes progression and epithelial-mesenchymal transition in renal cell carcinoma cells
    Chuang, Mei-Jen
    Sun, Kuang-Hui
    Tang, Shye-Jye
    Deng, Ming-Wei
    Wu, Yu-Hsin
    Sung, Jung-Sung
    Cha, Tai-Lung
    Sun, Guang-Huan
    [J]. CANCER SCIENCE, 2008, 99 (05) : 905 - 913
  • [6] Role for neutral sphingomyelinase-2 in tumor necrosis factor α-stimulated expression of vascular cell adhesion molecule-1 (VCAM) and intercellular adhesion molecule-1 (VCAM) in lung epithelial cells -: p38 MAPK is an upstream regulator of nSMase2
    Clarke, Christopher J.
    Truong, Thach-Giao
    Hannun, Yusuf A.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (02) : 1384 - 1396
  • [7] Sphingomyelin degradation is a key factor in dentin and bone mineralization:: Lessons from the fro/fro mouse
    Goldberg, M.
    Opsahl, S.
    Aubin, I.
    Septier, D.
    Chaussain-Miller, C.
    Boskey, A.
    Guenet, J. -L.
    [J]. JOURNAL OF DENTAL RESEARCH, 2008, 87 (01) : 9 - 13
  • [8] Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase
    Hofmann, K
    Tomiuk, S
    Wolff, G
    Stoffel, W
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) : 5895 - 5900
  • [9] Soluble expression and purification of synthetic human bone morphogenetic protein-2 in Escherichia coli
    Ihm, Hyo Jin
    Yang, Seung-Ju
    Huh, Jae-Wan
    Choi, Soo Young
    Cho, Sung-Woo
    [J]. BMB REPORTS, 2008, 41 (05) : 404 - 407
  • [10] Cbfβ regulates Runx2 function isoform-dependently in postnatal bone development
    Kanatani, Naoko
    Fujita, Takashi
    Fukuyama, Ryo
    Liu, Wenguang
    Yoshida, Carolina A.
    Moriishi, Takeshi
    Yamana, Kei
    Miyazaki, Toshihiro
    Toyosawa, Satoru
    Komori, Toshihisa
    [J]. DEVELOPMENTAL BIOLOGY, 2006, 296 (01) : 48 - 61