Multiple antigen peptide mimetics containing gp41 membrane-proximal external region elicit broad neutralizing antibodies against human immunodeficiency virus type 1 in guinea pigs

被引:10
作者
Zhang, Lishuang [1 ]
Miao, Liang [1 ]
Gong, Xin [1 ]
Zhang, Huayan [1 ]
Yang, Lan [1 ]
Shi, Yuhua [1 ]
Kong, Wei [1 ]
Jiang, Chunlai [1 ]
Shan, Yaming [1 ]
机构
[1] Jilin Univ, Coll Life Sci, Natl Engn Lab AIDS Vaccine, Changchun 130021, Peoples R China
关键词
broad neutralization; HIV-1; vaccine; MPER; multiple antigen peptide mimetics; HUMAN MONOCLONAL-ANTIBODY; HIV-1; GP41; ENVELOPE GLYCOPROTEIN; ECTODOMAIN REGION; VACCINE DESIGN; EPITOPE; FUSION; 4E10; CONFORMATION; 2F5;
D O I
10.1002/psc.2526
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eliciting a broadly neutralizing antibody response against the HIV-1 membrane-proximal external region (MPER) mimicking the activity of 4E10 and 2F5 monoclonal antibodies remains a major challenge. In this study, two novel tetra-branched peptide immunogens, 4E10- and 2F5-MAP4, were designed and synthesized using a MAP system. Guinea pigs were immunized with either of these two synthetic immunogens emulsified in an oil-phase adjuvant at 3-week intervals. After four immunizations, epitope-specific antibody responses were induced successfully, and moderate neutralizing activities against tier 1 (clades B, BC, AE) and tier 2 (clade C) HIV-1 pseudoviruses were detectable in unfractionated sera and purified IgGs. The synthetic gp41 membrane-proximal external region peptide mimetics, 4E10- and 2F5-MAP4, assisted by an appropriate adjuvant, are promising prophylactic vaccine candidates potentially capable of eliciting broadly neutralizing antibody responses against HIV-1. Copyright (c) 2013 European Peptide Society and John Wiley & Sons, Ltd.
引用
收藏
页码:491 / 498
页数:8
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