Separation of mono-, di-, and triphosphate nucleotides by cytosine substituted, silica-bound sapphyrin solid supports

A cytosine-substituted sapphyrin when used as a silica gel bound HPLC solid support effectively separates guanosine 5'-mono-, di-, and triphosphate from a mixture of the mono-, di- and triphosphates of cytidine, uridine, adenosine, and guanosine, respectively under isocratic conditions at pH 7. In addition, using this same solid support, cytidine 5'-monophosphate, guanosine 5'-monophosphate, adenosine 5'-monophosphate and uridine 5'-monophosphate are all readily separated from each other under similar HPLC conditions.