Defense Responses in Grapevine (cv. Mourvedre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering

被引:29
作者
Spagnolo, Alessandro [1 ]
Mondello, Vincenzo [1 ]
Larignon, Philippe [2 ]
Villaume, Sandra [1 ]
Rabenoelina, Fanja [1 ]
Clement, Christophe [1 ]
Fontaine, Florence [1 ]
机构
[1] Univ Reims, Struct Federat Rech SFR Condorcet FR CNRS 3417, Lab Stress Def & Reprod Plantes, Unite Rech Vignes & Vins Champagne EA 4707, BP 1039, F-51687 Reims 2, France
[2] Inst Francais Vigne & Vin Pole Rhone Mediterranee, 7 Ave Cazeaux, F-30230 Rodilhan, France
关键词
Botryosphaeria dieback; Neofusicoccum parvum; Diplodia seriata; defense responses; flowering; VITIS-VINIFERA L; PRIMARY METABOLISM; PLANT DEFENSE; ESCA PROPER; EXPRESSION; LEAF; CHARDONNAY; SYMPTOMS; PATTERNS; FORM;
D O I
10.3390/ijms18020393
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As a result of the increasing economic impact of grapevine trunk diseases on viticulture worldwide, efficient and viable control strategies are urgently needed. However, understanding both plant-pathogen interactions and plant physiological changes related to these diseases is fundamental to such an achievement. In this study, we analyzed the effect of inoculation with the Botryosphaeria dieback fungal agents, Neofusicoccum parvum and Diplodia seriata, with and without inflorescence removal at the onset of G stage (separated clusters), I stage (flowering) and M stage (veraison). A measure of lesion size and real-time reverse-transcription polymerase chain reaction-based analysis were carried out. The results clearly show the importance of inflorescences in the development of lesions associated with Botryosphaeria dieback pathogens inoculated on green stems of adult vines, especially at the onset of flowering. At flowering, the biggest necroses were observed with the inflorescences present, as well as an activation of the studied defense responses. Thus, an ineffective response to the pathogen could be consistent with a possible metabolic reprogramming linked to the host phenophase.
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页数:12
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