Mycobacterium tuberculosis DNA Detection Using Surface Plasmon Resonance Modulated by Telecommunication Wavelength

被引：12

作者：

Hsu, Shih-Hsiang ^{[1
]}

Lin, Yan-Yu ^{[1
]}

Lu, Shao-Hsi ^{[1
]}

Tsai, I-Fang ^{[2
]}

Lu, Yen-Ta ^{[3
,4
]}

Ho, Hsin-Tsung ^{[4
,5
]}

机构：

[1] Natl Taiwan Univ Sci & Technol, Dept Elect Engn, Taipei 10607, Taiwan

[2] Mackay Mem Hosp, Dept Med Res, Taipei 25160, Taiwan

[3] Mackay Mem Hosp, Dept Internal Med, Div Chest Med, Taipei 10449, Taiwan

[4] Mackay Med Coll, Dept Med, New Taipei City 25245, Taiwan

[5] Mackay Mem Hosp, Dept Lab Med, Taipei 25160, Taiwan

来源：

SENSORS | 2014年 / 14卷 / 01期

关键词：

Mycobacterium tuberculosis; surface plasmon resonance; wavelength modulation; SENSITIVITY-ENHANCEMENT; SENSOR; PCR;

D O I：

10.3390/s140100458

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 mu g/mL and 10 mu g/mL were successfully demonstrated to have the same spectral half-width in the dip for optimum coupling. The sensitivity was shown to be -0.087 dB/(mu g/mL) at all applied telecommunication wavelengths and the highest sensitivity achieved was 115 ng/mL without thiolated DNA immobilization onto a gold plate, which is better than the sensor limit of 400 ng/mL possible with commercial biosensor equipment.

引用

页码：458 / 467

页数：10

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