Chromatographic behaviour and lipophilicity of estradiol derivatives

被引:4
作者
Acanski, MM [1 ]
机构
[1] Univ Novi Sad, Fac Technol, Dept Appl & Engn Chem, Novi Sad 21000, Serbia Monteneg
关键词
column liquid chromatography; retention mechanism; correlation analysis; estradiol derivatives;
D O I
10.1365/s10337-005-0636-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The retention behaviour of estradiol derivatives has been studied by HPLC on polar chemically bonded stationary phases: C3CN, DIOL and C3NH2, commercially available columns. The mobile phases used were: methanol-water and acetonitrile-water in various proportions. Reversed-phase chromatography occurred on polar chemically bonded stationary phases. Correlation between the retention constants of estradiol derivatives obtained on polar chemically bonded phases and log P calculated via different methods was examined too.
引用
收藏
页码:475 / 482
页数:8
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