Incorporation and localisation of ganglioside GM3 in human intimal atherosclerotic lesions

Immunohistochemical examination showed that sections of intimal atherosclerotic plaques contained cells and cell of extracellular matrix specifically stained with antibodies against ganglioside GM3. No immunohistochemical staining was observed in areas bordering the plaques where there was no histological evidence of atherosclerosis, To determine whether the ganglioside GM3 deposits in the intimal plaques derived directly from plasma or were synthesised by intimal cells, intimal plaque and plasma LDL were assayed for ganglioside GM3 fatty acid composition. This assay showed that more than 50% of the fatty acids of GM3 isolated from both atherosclerotic and normal intima are either minor fatty acids or those absent from LDL GM3, We conclude that the GM3 deposits present in intimal plaque arise in intimal cells and do not derive from plasma LDL. (C) 1997 Elsevier Science B.V.