Gastric Tissue Damage Analysis Generated by Ischemia: Bioimpedance, Confocal Endomicroscopy, and Light Microscopy

被引：9
作者：
Beltran, Nohra E. [1 ,2 ]
Garcia, Laura E. [3 ]
Garcia-Lorenzana, Andmario [4 ]
机构：
[1] Univ Autonoma Metropolitana, Dept Proc & Tecnol, Mexico City 01120, DF, Mexico
[2] Univ Autonoma Metropolitana Iztapalapa, Ctr Nacl Invest Instrumentac & Imagenol Med, Mexico City 09340, DF, Mexico
[3] Univ Autonoma Metropolitana Iztapalapa, Dept Ingn Elect, Mexico City 09340, DF, Mexico
[4] Univ Autonoma Metropolitana Iztapalapa, Dept Reprod Biol, Mexico City 09340, DF, Mexico
来源：
BIOMED RESEARCH INTERNATIONAL | 2013年 / 2013卷
关键词：
IMPEDANCE SPECTROSCOPY; INTRAMUCOSAL PH; OXYGENATION; MORTALITY;
D O I：
10.1155/2013/824682
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The gastric mucosa ischemic tissular damage plays an important role in critical care patients' outcome, because it is the first damaged tissue by compensatory mechanism during shock. The aim of the study is to relate bioimpedance changes with tissular damage level generated by ischemia by means of confocal endomicroscopy and light microscopy. Bioimpedance of the gastric mucosa and confocal images were obtained from Wistar male rats during basal and ischemia conditions. They were anesthetized, and stain was applied (fluorescein and/or acriflavine). The impedance spectroscopy catheter was inserted and then confocal endomicroscopy probe. After basal measurements and biopsy, hepatic and gastric arteries clamping induced ischemia. Finally, pyloric antrum tissue was preserved in buffered formaldehyde (10%) for histology processing using light microscopy. Confocal images were equalized, binarized, and boundary defined, and infiltrations were quantified. Impedance and infiltrations increased with ischemia showing significant changes between basal and ischemia conditions (P < 0.01). Light microscopy analysis allows detection of general alterations in cellular and tissular integrity, confirming gastric reactance and confocal images quantification increments obtained during ischemia.
引用
收藏
页数：8
相关论文
共 24 条
[1]   TONOMETRY TO ASSESS THE ADEQUACY OF SPLANCHNIC OXYGENATION IN THE CRITICALLY ILL PATIENT [J].
ARNOLD, J ;
HENDRIKS, J ;
INCE, C ;
BRUINING, H .
INTENSIVE CARE MEDICINE, 1994, 20 (06) :452-456
[2]   The predictive value of gastric reactance for postoperative morbidity and mortality in cardiac surgery patients [J].
Beltran, N. E. ;
Sanchez-Miranda, G. ;
Godinez, M. M. ;
Diaz, U. ;
Sacristan, E. .
PHYSIOLOGICAL MEASUREMENT, 2010, 31 (11) :1423-1436
[3]   Gastric impedance spectroscopy in elective cardiovascular surgery patients [J].
Beltran, NE ;
Sanchez-Miranda, G ;
Godinez, M ;
Diaz, U ;
Sacristan, E .
PHYSIOLOGICAL MEASUREMENT, 2006, 27 (03) :265-277
[4]  
Beltran NE, 2010, US Patent Application, Patent No. [US2010/0268110 Al, 20100268110]
[5]  
Boutilier RG, 2001, J EXP BIOL, V204, P3171
[6]   Effects of hemorrhage on gastrointestinal oxygenation [J].
Dubin, A ;
Estenssoro, E ;
Murias, G ;
Canales, H ;
Sottile, P ;
Badie, J ;
Barán, M ;
Pálizas, F ;
Laporte, M ;
Díaz, MR .
INTENSIVE CARE MEDICINE, 2001, 27 (12) :1931-1936
[7]   ROLE OF CELL SWELLING IN ISCHEMIC RENAL DAMAGE AND PROTECTIVE EFFECT OF HYPERTONIC SOLUTE [J].
FLORES, J ;
BECK, CH ;
LEAF, A ;
DIBONA, DR .
JOURNAL OF CLINICAL INVESTIGATION, 1972, 51 (01) :118-&
[8]   Impedance spectroscopy on living tissue for determination of the state of organs [J].
Gersing, E .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1998, 45 (02) :145-149
[9]   In-vivo confocal real-time mini-microscopy in animal models of human inflammatory and neoplastic diseases [J].
Goetz, M. ;
Fottner, C. ;
Schirrmacher, E. ;
Delaney, P. ;
Gregor, S. ;
Schneider, C. ;
Strand, D. ;
Kanzler, S. ;
Memadathil, B. ;
Weyand, E. ;
Holtmann, M. ;
Schirrmacher, R. ;
Weber, M. M. ;
Anlauf, M. ;
Kloeppel, G. ;
Vieth, M. ;
Galle, P. R. ;
Bartenstein, P. ;
Neurath, M. F. ;
Kiesslich, R. .
ENDOSCOPY, 2007, 39 (04) :350-356
[10]  
Granger D. N., 1988, AM J PHYSL, V255, p[24, 6]
123