First draft genome sequence of the Japanese eel, Anguilla japonica

被引:87
作者
Henkel, Christiaan V. [1 ]
Dirks, Ron P. [1 ]
de Wijze, Danielle L. [2 ]
Minegishi, Yuki [2 ]
Aoyama, Jun [3 ]
Jansen, Hans J. [1 ]
Turner, Ben [4 ]
Knudsen, Bjarne [4 ]
Bundgaard, Martin [4 ]
Hvam, Kenneth Lyneborg [4 ]
Boetzer, Marten [5 ]
Pirovano, Walter [5 ]
Weltzien, Finn-Arne [6 ]
Dufour, Sylvie [7 ]
Tsukamoto, Katsumi [3 ]
Spaink, Herman P. [2 ]
van den Thillart, Guido E. E. J. M. [2 ]
机构
[1] ZF Screens BV, NL-2333 CA Leiden, Netherlands
[2] Leiden Univ, Inst Biol, NL-2300 RA Leiden, Netherlands
[3] Univ Tokyo, Ocean Res Inst, Tokyo 2778564, Japan
[4] CLC Bio, DK-8200 Aarhus N, Denmark
[5] BaseClear BV, NL-2333 CC Leiden, Netherlands
[6] Norwegian Sch Vet Sci, N-0033 Oslo, Norway
[7] Museum Natl Hist Nat, CNRS 7208, UMR BOREA, F-75231 Paris 05, France
关键词
Eel; Anguilla; Assembly pipeline; Illumina sequencing; De novo genome; Hox genes; MATURATION; FERTILIZATION; ANNOTATION;
D O I
10.1016/j.gene.2012.09.064
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The Japanese eel is a much appreciated research object and very important for Asian aquaculture; however, its genomic resources are still limited. We have used a streamlined bioinformatics pipeline for the de novo assembly of the genome sequence of the Japanese eel from raw Illumina sequence reads. The total assembled genome has a size of 1.15 Gbp, which is divided over 323,776 scaffolds with an N50 of 52,849 bp, a minimum scaffold size of 200 bp and a maximum scaffold size of 1.14 Mbp. Direct comparison of a representative set of scaffolds revealed that all the Hox genes and their intergenic distances are almost perfectly conserved between the European and the Japanese eel. The first draft genome sequence of an organism strongly catalyzes research progress in multiple fields. Therefore, the Japanese eel genome sequence will provide a rich resource of data for all scientists working on this important fish species. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:195 / 201
页数:7
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