PIXE analysis of Zn enzymes

被引：10

作者：

Solís, C

Oliver, A

Andrade, E

Ruvalcaba-Sil, JL

Romero, I

Celis, H

机构：

[1] Univ Nacl Autonoma Mexico, Inst Fis, Mexico City 01000, DF, Mexico

[2] Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico

[3] Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 01000, DF, Mexico

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 1999年 / 150卷 / 1-4期

关键词：

PIXE; zinc; enzymes; electrophoresis;

D O I：

10.1016/S0168-583X(98)01012-X

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Zinc is a necessary component in the action and structural stability of many enzymes, Some of them are well characterized, but in others, Zn stoichiometry and its association is not known, PIXE has been proven to be a suitable technique for analyzing metallic proteins embedded in electrophoresis gels. In this study, PIXE has been used to investigate the Zn content of enzymes that are known to carry Zn atoms. These include the carbonic anhydrase, an enzyme well characterized by other methods and the cytoplasmic pyrophosphatase of Rhodospirillum rubrum that is known to require Zn to be stable but not how many metal inns are involved or how they are bound to the enzyme, Native proteins have been purified by polyacrylamide gel electrophoresis and direct identification and quantification of Zn in the gel bands was performed with an external proton beam of 3.7 MeV energy. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：222 / 225

页数：4

共 11 条

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